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Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus

التفاصيل البيبلوغرافية
العنوان: Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus
المؤلفون: Ajaya K. Biswal, Melani A. Atmodjo, Sivakumar Pattathil, Robert A. Amos, Xiaohan Yang, Kim Winkeler, Cassandra Collins, Sushree S. Mohanty, David Ryno, Li Tan, Ivana Gelineo-Albersheim, Kimberly Hunt, Robert W. Sykes, Geoffrey B. Turner, Angela Ziebell, Mark F. Davis, Stephen R. Decker, Michael G. Hahn, Debra Mohnen
المصدر: Biotechnology for Biofuels, Vol 11, Iss 1, Pp 1-26 (2018)
بيانات النشر: BMC, 2018.
سنة النشر: 2018
المجموعة: LCC:Fuel
LCC:Biotechnology
مصطلحات موضوعية: Plant cell wall, Biofuel, Biomass, Pectin, Xylan, Yield, Fuel, TP315-360, Biotechnology, TP248.13-248.65
الوصف: Abstract Background The development of fast-growing hardwood trees as a source of lignocellulosic biomass for biofuel and biomaterial production requires a thorough understanding of the plant cell wall structure and function that underlie the inherent recalcitrance properties of woody biomass. Downregulation of GAUT12.1 in Populus deltoides was recently reported to result in improved biomass saccharification, plant growth, and biomass yield. To further understand GAUT12.1 function in biomass recalcitrance and plant growth, here we report the effects of P. trichocarpa GAUT12.1 overexpression in P. deltoides. Results Increasing GAUT12.1 transcript expression by 7–49% in P. deltoides PtGAUT12.1-overexpression (OE) lines resulted in a nearly complete opposite biomass saccharification and plant growth phenotype to that observed previously in PdGAUT12.1-knockdown (KD) lines. This included significantly reduced glucose, xylose, and total sugar release (12–13%), plant height (6–54%), stem diameter (8–40%), and overall total aerial biomass yield (48–61%) in 3-month-old, greenhouse-grown PtGAUT12.1-OE lines compared to controls. Total lignin content was unaffected by the gene overexpression. Importantly, selected PtGAUT12.1-OE lines retained the recalcitrance and growth phenotypes upon growth for 9 months in the greenhouse and 2.8 years in the field. PtGAUT12.1-OE plants had significantly smaller leaves with lower relative water content, and significantly reduced stem wood xylem cell numbers and size. At the cell wall level, xylose and galacturonic acid contents increased markedly in total cell walls as well as in soluble and insoluble cell wall extracts, consistent with increased amounts of xylan and homogalacturonan in the PtGAUT12.1-OE lines. This led to increased cell wall recalcitrance, as manifested by the 9–15% reduced amounts of recovered extractable wall materials and 8–15% greater amounts of final insoluble pellet in the PtGAUT12.1-OE lines compared to controls. Conclusions The combined phenotype and chemotype data from P. deltoides PtGAUT12.1-OE and PdGAUT12.1-KD transgenics clearly establish GAUT12.1 as a recalcitrance- and growth-associated gene in poplar. Overall, the data support the hypothesis that GAUT12.1 synthesizes either an HG-containing primer for xylan synthesis or an HG glycan required for proper xylan deposition, anchoring, and/or architecture in the wall, and the possibility of HG and xylan glycans being connected to each other by a base-sensitive covalent linkage.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1754-6834
Relation: http://link.springer.com/article/10.1186/s13068-017-1002-y; https://doaj.org/toc/1754-6834
DOI: 10.1186/s13068-017-1002-y
URL الوصول: https://doaj.org/article/0844b9bfe7c240d49535ed1bf218ce65
رقم الأكسشن: edsdoj.0844b9bfe7c240d49535ed1bf218ce65
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:17546834
DOI:10.1186/s13068-017-1002-y