دورية أكاديمية
Inhibition of NLRP3 attenuates sodium dextran sulfate-induced inflammatory bowel disease through gut microbiota regulation
| العنوان: | Inhibition of NLRP3 attenuates sodium dextran sulfate-induced inflammatory bowel disease through gut microbiota regulation |
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| المؤلفون: | Shi-Le Wang, Man-Man Zhang, Han Zhou, Guo-Qiang Su, Yi Ding, Guang-Hui Xu, Xu Wang, Cheng-Fu Li, Wei-Feng Huang, Li-Tao Yi |
| المصدر: | Biomedical Journal, Vol 46, Iss 5, Pp 100580- (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | NLRP3, Inflammatory bowel disease, Microbiota, Cytokine, Dextran sulfate sodium, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Background: Inflammatory bowel disease (IBD) is a chronic, life-threatening inflammatory disease of gastrointestinal tissue characterized by inflammation of the gut. Recent studies have shown that gut microbiota is involved in the pathophysiology of IBD. However, it is unknown whether direct inhibition of NLR family pyrin domain containing 3 (NLRP3) inflammasome regulates IBD and alters gut microbiota. Methods: Here, the NLRP3 expression was evaluated in the colon of IBD subjects. Then, we investigated the effects of NLRP3 inhibition by MCC950 on the gut microbiota and IBD-like symptoms induced by dextran sulfate sodium (DSS). Results: Firstly, NLRP3 and IL-1β levels were increased in patients with IBD as compared with healthy individuals. Then, the animal experiment showed that NLRP3 inhibition by MCC950 significantly attenuated IBD-like symptoms such as diarrhea and colonic inflammation in DSS-induced mice. In addition, NLRP3 inhibition inhibited NLRP3/ASC/caspase-1/IL-1β signaling pathway in the colon, which was over-activated by DSS. Furthermore, MCC950 increased the abundance of phylum Firmicutes, decreased the abundance of phylum Bacteroidetes, and increased the Firmicutes/Bacteroidetes ratio, indicating that the inhibition of NLRP3 inflammasome could regulate the abundance of intestinal flora. According to correlation analysis, NLRP3 might produce its functional role in the regulation of oxidation indicators by changing the gut microbiota composition, especially the phylum Bacteroidota, genus Lactobacillus and species Lactobacillus reuteri. Conclusions: This study suggests that NLRP3 inflammasome inhibition attenuates IBD-like symptoms by regulating gut microbiota, and provides a basis for the clinical application of NLRP3 as a target for the treatment of IBD. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2319-4170 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2319417023000045; https://doaj.org/toc/2319-4170 |
| DOI: | 10.1016/j.bj.2023.01.004 |
| URL الوصول: | https://doaj.org/article/d092e1cd4fd2405192018d47d62b7786 |
| رقم الأكسشن: | edsdoj.092e1cd4fd2405192018d47d62b7786 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23194170 |
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| DOI: | 10.1016/j.bj.2023.01.004 |