دورية أكاديمية
Remodeling of Lipid A in Pseudomonas syringae pv. phaseolicola In Vitro
| العنوان: | Remodeling of Lipid A in Pseudomonas syringae pv. phaseolicola In Vitro |
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| المؤلفون: | Tim Gerster, Michelle Wröbel, Casey E. Hofstaedter, Dominik Schwudke, Robert K. Ernst, Stefanie Ranf, Nicolas Gisch |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 4, p 1996 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | lipid A, lipopolysaccharide, Pseudomonas, lipopolysaccharide remodeling, mass spectrometry, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Pseudomonas species infect a variety of organisms, including mammals and plants. Mammalian pathogens of the Pseudomonas family modify their lipid A during host entry to evade immune responses and to create an effective barrier against different environments, for example by removal of primary acyl chains, addition of phosphoethanolamine (P-EtN) to primary phosphates, and hydroxylation of secondary acyl chains. For Pseudomonas syringae pv. phaseolicola (Pph) 1448A, an economically important pathogen of beans, we observed similar lipid A modifications by mass spectrometric analysis. Therefore, we investigated predicted proteomes of various plant-associated Pseudomonas spp. for putative lipid A-modifying proteins using the well-studied mammalian pathogen Pseudomonas aeruginosa as a reference. We generated isogenic mutant strains of candidate genes and analyzed their lipid A. We show that the function of PagL, LpxO, and EptA is generally conserved in Pph 1448A. PagL-mediated de-acylation occurs at the distal glucosamine, whereas LpxO hydroxylates the secondary acyl chain on the distal glucosamine. The addition of P-EtN catalyzed by EptA occurs at both phosphates of lipid A. Our study characterizes lipid A modifications in vitro and provides a useful set of mutant strains relevant for further functional studies on lipid A modifications in Pph 1448A. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/23/4/1996; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms23041996 |
| URL الوصول: | https://doaj.org/article/097013c7953f495db041afbfe2e23c22 |
| رقم الأكسشن: | edsdoj.097013c7953f495db041afbfe2e23c22 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms23041996 |