دورية أكاديمية
Infrared Characterization of the Bidirectional Oxygen-Sensitive [NiFe]-Hydrogenase from E. coli
| العنوان: | Infrared Characterization of the Bidirectional Oxygen-Sensitive [NiFe]-Hydrogenase from E. coli |
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| المؤلفون: | Moritz Senger, Konstantin Laun, Basem Soboh, Sven T. Stripp |
| المصدر: | Catalysts, Vol 8, Iss 11, p 530 (2018) |
| بيانات النشر: | MDPI AG, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Chemical technology LCC:Chemistry |
| مصطلحات موضوعية: | redox enzymes, FTIR spectroscopy, small molecules, Chemical technology, TP1-1185, Chemistry, QD1-999 |
| الوصف: | [NiFe]-hydrogenases are gas-processing metalloenzymes that catalyze the conversion of dihydrogen (H2) to protons and electrons in a broad range of microorganisms. Within the framework of green chemistry, the molecular proceedings of biological hydrogen turnover inspired the design of novel catalytic compounds for H2 generation. The bidirectional “O2-sensitive„ [NiFe]-hydrogenase from Escherichia coli HYD-2 has recently been crystallized; however, a systematic infrared characterization in the presence of natural reactants is not available yet. In this study, we analyze HYD-2 from E. coli by in situ attenuated total reflection Fourier-transform infrared spectroscopy (ATR FTIR) under quantitative gas control. We provide an experimental assignment of all catalytically relevant redox intermediates alongside the O2- and CO-inhibited cofactor species. Furthermore, the reactivity and mutual competition between H2, O2, and CO was probed in real time, which lays the foundation for a comparison with other enzymes, e.g., “O2-tolerant„ [NiFe]-hydrogenases. Surprisingly, only Ni-B was observed in the presence of O2 with no indications for the “unready„ Ni-A state. The presented work proves the capabilities of in situ ATR FTIR spectroscopy as an efficient and powerful technique for the analysis of biological macromolecules and enzymatic small molecule catalysis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2073-4344 |
| Relation: | https://www.mdpi.com/2073-4344/8/11/530; https://doaj.org/toc/2073-4344 |
| DOI: | 10.3390/catal8110530 |
| URL الوصول: | https://doaj.org/article/cd09c6b284574e968f337c5b74bb0c0e |
| رقم الأكسشن: | edsdoj.09c6b284574e968f337c5b74bb0c0e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20734344 |
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| DOI: | 10.3390/catal8110530 |