دورية أكاديمية
Metabolism characterization and toxicity of N-hydap, a marine candidate drug for lung cancer therapy by LC–MS method
| العنوان: | Metabolism characterization and toxicity of N-hydap, a marine candidate drug for lung cancer therapy by LC–MS method |
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| المؤلفون: | Jindi Lu, Weimin Liang, Yiwei Hu, Xi Zhang, Ping Yu, Meiqun Cai, Danni Xie, Qiong Zhou, Xuefeng Zhou, Yonghong Liu, Junfeng Wang, Jiayin Guo, Lan Tang |
| المصدر: | Natural Products and Bioprospecting, Vol 14, Iss 1, Pp 1-21 (2024) |
| بيانات النشر: | SpringerOpen, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Botany |
| مصطلحات موضوعية: | N-Hydap, Metabolism, Pharmacokinetics, DMEs, Toxicity, DDIs, Botany, QK1-989 |
| الوصف: | Abstract N-Hydroxyapiosporamide (N-hydap), a marine product derived from a sponge-associated fungus, has shown promising inhibitory effects on small cell lung cancer (SCLC). However, there is limited understanding of its metabolic pathways and characteristics. This study explored the in vitro metabolic profiles of N-hydap in human recombinant cytochrome P450s (CYPs) and UDP-glucuronosyltransferases (UGTs), as well as human/rat/mice microsomes, and also the pharmacokinetic properties by HPLC–MS/MS. Additionally, the cocktail probe method was used to investigate the potential to create drug-drug interactions (DDIs). N-Hydap was metabolically unstable in various microsomes after 1 h, with about 50% and 70% of it being eliminated by CYPs and UGTs, respectively. UGT1A3 was the main enzyme involved in glucuronidation (over 80%), making glucuronide the primary metabolite. With a favorable bioavailability of 24.0%, N-hydap exhibited a higher distribution in the lungs (26.26%), accounting for its efficacy against SCLC. Administering N-hydap to mice at normal doses via gavage did not result in significant toxicity. Furthermore, N-hydap was found to affect the catalytic activity of drug metabolic enzymes (DMEs), particularly increasing the activity of UGT1A3, suggesting potential for DDIs. Understanding the metabolic pathways and properties of N-hydap should improve our knowledge of its drug efficacy, toxicity, and potential for DDIs. Graphical Abstract |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2192-2195 2192-2209 |
| Relation: | https://doaj.org/toc/2192-2195; https://doaj.org/toc/2192-2209 |
| DOI: | 10.1007/s13659-024-00455-x |
| URL الوصول: | https://doaj.org/article/0a9335983ec541d489b4bb865454e5fe |
| رقم الأكسشن: | edsdoj.0a9335983ec541d489b4bb865454e5fe |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 21922195 21922209 |
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| DOI: | 10.1007/s13659-024-00455-x |