دورية أكاديمية
Ca2+-Permeable Channels/Ca2+ Signaling in the Regulation of Ileal Na+/Gln Co-Transport in Mice
| العنوان: | Ca2+-Permeable Channels/Ca2+ Signaling in the Regulation of Ileal Na+/Gln Co-Transport in Mice |
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| المؤلفون: | Fenglan Chu, Hanxing Wan, Weidong Xiao, Hui Dong, Muhan Lü |
| المصدر: | Frontiers in Pharmacology, Vol 13 (2022) |
| بيانات النشر: | Frontiers Media S.A., 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | calcium signaling, VGCC, SOCE, CICR, TRPV, NCX, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Oral glutamine (Gln) has been widely used in gastrointestinal (GI) clinical practice, but it is unclear if Ca2+ regulates intestinal Gln transport, although both of them are essential nutrients for mammals. Chambers were used to determine Gln (25 mM)-induced Isc through Na+/Gln co-transporters in the small intestine in the absence or the presence of selective activators or blockers of ion channels and transporters. Luminal but not serosal application of Gln induced marked intestinal Isc, especially in the distal ileum. Lowering luminal Na+ almost abolished the Gln-induced ileal Isc, in which the calcium-sensitive receptor (CaSR) activation were not involved. Ca2+ removal from both luminal and serosal sides of the ileum significantly reduced Gln- Isc. Blocking either luminal Ca2+ entry via the voltage-gated calcium channels (VGCC) or endoplasmic reticulum (ER) release via inositol 1,4,5-triphosphate receptor (IP3R) and ryanodine receptor (RyR) attenuated the Gln-induced ileal Isc, Likewise, blocking serosal Ca2+ entry via the store-operated Ca2+ entry (SOCE), TRPV1/2 channels, and Na+/Ca2+ exchangers (NCX) attenuated the Gln-induced ileal Isc. In contrast, activating TRPV1/2 channels enhanced the Gln-induced ileal Isc. We concluded that Ca2+ signaling is critical for intestinal Gln transport, and multiple plasma membrane Ca2+-permeable channels and transporters play roles in this process. The Ca2+ regulation of ileal Na+/Gln transport expands our understanding of intestinal nutrient uptake and may be significant in GI health and disease. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1663-9812 84575468 |
| Relation: | https://www.frontiersin.org/articles/10.3389/fphar.2022.816133/full; https://doaj.org/toc/1663-9812 |
| DOI: | 10.3389/fphar.2022.816133 |
| URL الوصول: | https://doaj.org/article/0ab648a84575468789653b3004856075 |
| رقم الأكسشن: | edsdoj.0ab648a84575468789653b3004856075 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16639812 84575468 |
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| DOI: | 10.3389/fphar.2022.816133 |