دورية أكاديمية
miR-205-5p inhibits human endometriosis progression by targeting ANGPT2 in endometrial stromal cells
| العنوان: | miR-205-5p inhibits human endometriosis progression by targeting ANGPT2 in endometrial stromal cells |
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| المؤلفون: | Chen-Fei Zhou, Min-Juan Liu, Wei Wang, Sha Wu, Yu-Xin Huang, Guo-Bin Chen, Li-Min Liu, Dong-Xian Peng, Xue-Feng Wang, Xu-Zi Cai, Xiao-Xuan Li, Wan-Qin Feng, Ying Ma |
| المصدر: | Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-13 (2019) |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Endometriosis, miR-205-5p, Endometrial stromal cells, ANGPT2, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Abstract Background miRNA expression profiles in ectopic endometrium (EC) serving as pathophysiologic genetic fingerprints contribute to determining endometriosis progression; however, the underlying molecular mechanisms remain unknown. Methods miRNA microarray analysis was used to determine the expression profiling of EC fresh tissues. qRT-PCR was performed to screen miR-205-5p expression in EC tissues. The roles of miR-205-5p and its candidate target gene, angiopoietin-2 (ANGPT2), in endometriosis progression were confirmed on the basis of both in vitro and in vivo systems. miR-205-5p and ANGPT2 expression were measured by in situ hybridization and immunochemistry, and their clinical significance was statistically analysed. Results miR-205-5p was screened as a novel suppressor of endometriosis through primary ectopic endometrial stromal cell migration, invasion, and apoptosis assay in vitro, along with endometrial-like xenograft growth and apoptosis in vivo. In addition, ANGPT2 was identified as a direct target of miR-205-5p through bioinformatic target prediction and luciferase reporter assay. Re-expression and knockdown of ANGPT2 could respectively rescue and simulate the effects induced by miR-205-5p. Importantly, the miR-205-5p-ANGPT2 axis was found to activate the ERK/AKT pathway in endometriosis. Finally, miR-205-5p and ANGPT2 expression were closely correlated with the endometriosis severity. Conclusion The newly identified miR-205-5p-ANGPT2-AKT/ERK axis illustrates the molecular mechanism of endometriosis progression and may represent a novel diagnostic biomarker and therapeutic target for disease treatment. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1757-6512 |
| Relation: | http://link.springer.com/article/10.1186/s13287-019-1388-5; https://doaj.org/toc/1757-6512 |
| DOI: | 10.1186/s13287-019-1388-5 |
| URL الوصول: | https://doaj.org/article/0b3216c51ab34d939d0eafd8ca0c64fc |
| رقم الأكسشن: | edsdoj.0b3216c51ab34d939d0eafd8ca0c64fc |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17576512 |
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| DOI: | 10.1186/s13287-019-1388-5 |