دورية أكاديمية
أعرض في EDS

Identification of microRNA that represses IRS-1 expression in liver.

التفاصيل البيبلوغرافية
العنوان: Identification of microRNA that represses IRS-1 expression in liver.
المؤلفون: Kaoru Ono, Motoyuki Igata, Tatsuya Kondo, Sayaka Kitano, Yuki Takaki, Satoko Hanatani, Masaji Sakaguchi, Rieko Goto, Takafumi Senokuchi, Junji Kawashima, Noboru Furukawa, Hiroyuki Motoshima, Eiichi Araki
المصدر: PLoS ONE, Vol 13, Iss 1, p e0191553 (2018)
بيانات النشر: Public Library of Science (PLoS), 2018.
سنة النشر: 2018
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: MicroRNAs (miRNAs) are short, non-coding RNAs that post-transcriptionally regulate gene expression and have been shown to participate in almost every cellular process. Several miRNAs have recently been implicated in glucose metabolism, but the roles of miRNAs in insulin-resistant conditions, such as obesity or type 2 diabetes, are largely unknown. Herein, we focused on miR-222, the expression of which was increased in the livers of high fat/high sucrose diet-fed mice injected with gold thioglucose (G+HFHSD). Overexpression of miR-222 in primary mouse hepatocytes attenuated Akt phosphorylation induced by insulin, indicating that miR-222 negatively regulates insulin signaling. As per in silico analysis, miR-222 potentially binds to the 3' untranslated region (3' UTR) of the IRS-1 gene, a key insulin signaling molecule. In fact, IRS-1 protein expression was decreased in the livers of G+HFHSD-fed mice. We further confirmed a direct interaction between miR-222 and the 3' UTR of IRS-1 via luciferase assays. Our findings suggest that up-regulation of miR-222 followed by reduction in IRS-1 expression may be a viable mechanism of insulin resistance in the liver.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC5783395?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0191553
URL الوصول: https://doaj.org/article/a0b375491a1f47e983b79093799e080b
رقم الأكسشن: edsdoj.0b375491a1f47e983b79093799e080b
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0191553