دورية أكاديمية
Insights into Early Onset Colorectal Cancer through Analysis of Normal Colon Organoids of Familial Adenomatous Polyposis Patients
| العنوان: | Insights into Early Onset Colorectal Cancer through Analysis of Normal Colon Organoids of Familial Adenomatous Polyposis Patients |
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| المؤلفون: | Matthew A. Devall, Stephen Eaton, Mourad W. Ali, Steven M. Powell, Li Li, Graham Casey |
| المصدر: | Cancers, Vol 14, Iss 17, p 4138 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | familial adenomatous polyposis, early onset colorectal cancer, colon organoid, RNA-seq, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Early onset colorectal cancer (EOCRC) rates have increased in recent decades. While lowering the recommended age for routine colonoscopies to 45 may reduce this burden, such measures do not address those who develop CRC before that age. Additional measures are needed to identify individuals at-risk for CRC. To better define transcriptomic events that precede the development of CRC, we performed RNA-sequencing analysis in colon organoids derived from seven healthy and six familial adenomatous polyposis (FAP) patients. This led to the identification of 2635 significant differentially expressed genes (FDR < 0.05). Through secondary analysis of publicly available datasets, we found that these genes were enriched for significant genes also present in FAP CRC and non-hereditary CRC datasets, including a subset that were unique to EOCRC. By exposing FAP colon organoids to a three-day ethanol treatment, we found that two EOCRC-relevant genes were also targets of CRC related lifestyle factors. Our data provides unique insight into the potential, early mechanisms of CRC development in colon epithelial cells, which may provide biomarkers for patient monitoring. We also show how modifiable lifestyle factors may further alter genes relevant to EOCRC, adding weight to the hypothesis that such factors represent an important contributor to increased EOCRC incidence. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 14174138 2072-6694 |
| Relation: | https://www.mdpi.com/2072-6694/14/17/4138; https://doaj.org/toc/2072-6694 |
| DOI: | 10.3390/cancers14174138 |
| URL الوصول: | https://doaj.org/article/aa0b476bc30a45d8b6f298cce0288f36 |
| رقم الأكسشن: | edsdoj.0b476bc30a45d8b6f298cce0288f36 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14174138 20726694 |
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| DOI: | 10.3390/cancers14174138 |