دورية أكاديمية
Pharmacological evaluation of the anxiolytic-like effects of Lippia graveolens and bioactive compounds
| العنوان: | Pharmacological evaluation of the anxiolytic-like effects of Lippia graveolens and bioactive compounds |
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| المؤلفون: | María Eva González-Trujano, Laura Yunuen Hernández-Sánchez, Verónica Muñoz Ocotero, Alejandro Dorazco-González, Patricia Guevara Fefer, Eva Aguirre-Hernández |
| المصدر: | Pharmaceutical Biology, Vol 55, Iss 1, Pp 1569-1576 (2017) |
| بيانات النشر: | Taylor & Francis Group, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | anxiety, cirsimaritin, naringenin, mexican traditional medicine, terpenoid, verbenaceae, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Context: Lippia species (Verbenaceae) are widely used in Latin America and Africa as folk medicine for their tranquilizing properties. Objective: To evaluate the anxiolytic-like effects and safety of Lippia graveolens Kunth. by exploring its aqueous and organic leaf extracts and identifying the responsible chemical constituents. Material and methods: Aqueous and organic extracts (hexane, ethyl acetate and methanol) were pharmacologically evaluated at several doses. Chemical constituents were identified using MS, NMR and GC-MS analysis. The isolated compounds (3 mg/kg, i.p.), extracts (1, 3, 10 and 30 mg/kg, i.p.), and the reference drug diazepam (0.1 mg/kg, i.p.) were assessed in CD-1 mice using experimental behavioural models: open-field, cylinder, hole-board, plus-maze and sodium pentobarbital-induced hypnosis, as well as their acute toxicity (LD50). Results: After administration of the extracts and bioactive compounds, a significant anxiolytic-like response from 1 mg/kg, i.p. was observed, resembling the effect of diazepam. Major presence of thymol (33.40%) was observed in the hexane extract; whereas for the first time in this species a p-cymene + thymol mixture (9.78%), naringenin (0.18%) and cirsimaritin (1.16%) were obtained as bioactive constituents of the ethyl acetate crude extract. Acute toxicity was calculated to be LD50 = 1000 mg/kg for the crude hexane extract, lower in comparison to the other extracts analyzed (LD50 > 2000 mg/kg). Discussion and conclusion: Our results suggest that L. graveolens exerts anxiolytic-like activity involving many kinds of constituents, mainly of the terpenoid and flavonoid nature. These results reinforce the potential use of this species in the therapy of anxiety. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1388-0209 1744-5116 13880209 |
| Relation: | https://doaj.org/toc/1388-0209; https://doaj.org/toc/1744-5116 |
| DOI: | 10.1080/13880209.2017.1310908 |
| URL الوصول: | https://doaj.org/article/0b9b1036de324810af26f91fd60f3f6e |
| رقم الأكسشن: | edsdoj.0b9b1036de324810af26f91fd60f3f6e |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 13880209 17445116 |
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| DOI: | 10.1080/13880209.2017.1310908 |