دورية أكاديمية
F4-Neuroprostane Effects on Human Sperm
| العنوان: | F4-Neuroprostane Effects on Human Sperm |
|---|---|
| المؤلفون: | Elena Moretti, Cinzia Signorini, Daria Noto, Roberta Corsaro, Lucia Micheli, Thierry Durand, Camille Oger, Jean Marie Galano, Giulia Collodel |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 2, p 935 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | acrosome reaction, capacitation, F4-neuroprostanes, human sperm, in vitro study, sperm motility, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Swim-up selected human sperm were incubated with 7 ng F4-neuroprostanes (F4-NeuroPs) for 2 and 4 h. Sperm motility and membrane mitochondrial potential (MMP) were evaluated. The percentage of reacted acrosome was assessed by pisum sativum agglutinin (PSA). Chromatin integrity was detected using the acridine orange (AO) assay and localization of the ryanodine receptor was performed by immunofluorescence analysis. Sperm progressive motility (p = 0.02) and the percentage of sperm showing a strong MMP signal (p = 0.012) significantly increased after 2 h F4-NeuroP incubation compared to control samples. The AO assay did not show differences in the percentage of sperm with dsDNA between treated or control samples. Meanwhile, a significantly higher number of sperm with reacted acrosomes was highlighted by PSA localization after 4 h F4-NeuroP incubation. Finally, using an anti-ryanodine antibody, the immunofluorescence signal was differentially distributed at 2 and 4 h: a strong signal was evident in the midpiece and postacrosomal sheath (70% of sperm) at 2 h, whereas a dotted one appeared at 4 h (53% of sperm). A defined concentration of F4-NeuroPs in seminal fluid may induce sperm capacitation via channel ions present in sperm cells, representing an aid during in vitro sperm preparation that may increase the positive outcome of assisted fertilization. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/24/2/935; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms24020935 |
| URL الوصول: | https://doaj.org/article/c0bcffc18cf34165889cf3e6dd3cbe8d |
| رقم الأكسشن: | edsdoj.0bcffc18cf34165889cf3e6dd3cbe8d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
|---|---|
| DOI: | 10.3390/ijms24020935 |