دورية أكاديمية
Transcriptome profiling of subepithelial PDGFRα cells in colonic mucosa reveals several cell-selective markers
| العنوان: | Transcriptome profiling of subepithelial PDGFRα cells in colonic mucosa reveals several cell-selective markers |
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| المؤلفون: | Se Eun Ha, Byungchang Jin, Brian G. Jorgensen, Hannah Zogg, Lai Wei, Rajan Singh, Chanjae Park, Masaaki Kurahashi, Sei Kim, Gain Baek, Sandra M. Poudrier, Moon Young Lee, Kenton M. Sanders, Seungil Ro |
| المصدر: | PLoS ONE, Vol 17, Iss 5 (2022) |
| بيانات النشر: | Public Library of Science (PLoS), 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Subepithelial platelet-derived growth factor receptor alpha (PDGFRα)+ cells found in the colonic mucosal tissue come in close contact with epithelial cells, immune cells, neurons, capillaries, and lymphatic networks. Mucosal subepithelial PDGFRα+ cells (MuPαC) are important regulators in various intestinal diseases including fibrosis and inflammation. However, the transcriptome of MuPαC has not yet been elucidated. Using Pdgfra-eGFP mice and flow cytometry, we isolated colonic MuPαC and obtained their transcriptome data. In analyzing the transcriptome, we identified three novel, and selectively expressed, markers (Adamdec1, Fin1, and Col6a4) found in MuPαC. In addition, we identified a unique set of MuPαC-enriched genetic signatures including groups of growth factors, transcription factors, gap junction proteins, extracellular proteins, receptors, cytokines, protein kinases, phosphatases, and peptidases. These selective groups of genetic signatures are linked to the unique cellular identity and function of MuPαC. Furthermore, we have added this MuPαC transcriptome data to our Smooth Muscle Genome Browser that contains the transcriptome data of jejunal and colonic smooth muscle cells (SMC), interstitial cells of Cajal (ICC), and smooth muscle resident PDGFRα+ cells: (https://med.unr.edu/physio/transcriptome). This online resource provides a comprehensive reference of all currently known genetic transcripts expressed in primary MuPαC in the colon along with smooth muscle resident PDGFRα cells, SMC, and ICC in the murine colon and jejunum. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106222/?tool=EBI; https://doaj.org/toc/1932-6203 |
| URL الوصول: | https://doaj.org/article/e0ce6f5c5dfc4d18818052ef84064da0 |
| رقم الأكسشن: | edsdoj.0ce6f5c5dfc4d18818052ef84064da0 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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