دورية أكاديمية
A putative ATPase mediates RNA transcription and capping in a dsRNA virus
العنوان: | A putative ATPase mediates RNA transcription and capping in a dsRNA virus |
---|---|
المؤلفون: | Xuekui Yu, Jiansen Jiang, Jingchen Sun, Z Hong Zhou |
المصدر: | eLife, Vol 4 (2015) |
بيانات النشر: | eLife Sciences Publications Ltd, 2015. |
سنة النشر: | 2015 |
المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
مصطلحات موضوعية: | viral ATPase, histidine-mediated guanylyl transfer, allosteric regulation, conformational change, Medicine, Science, Biology (General), QH301-705.5 |
الوصف: | mRNA transcription in dsRNA viruses is a highly regulated process but the mechanism of this regulation is not known. Here, by nucleoside triphosphatase (NTPase) assay and comparisons of six high-resolution (2.9–3.1 Å) cryo-electron microscopy structures of cytoplasmic polyhedrosis virus with bound ligands, we show that the large sub-domain of the guanylyltransferase (GTase) domain of the turret protein (TP) also has an ATP-binding site and is likely an ATPase. S-adenosyl-L-methionine (SAM) acts as a signal and binds the methylase-2 domain of TP to induce conformational change of the viral capsid, which in turn activates the putative ATPase. ATP binding/hydrolysis leads to an enlarged capsid for efficient mRNA synthesis, an open GTase domain for His217-mediated guanylyl transfer, and an open methylase-1 domain for SAM binding and methyl transfer. Taken together, our data support a role of the putative ATPase in mediating the activation of mRNA transcription and capping within the confines of the virus. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2050-084X |
Relation: | https://elifesciences.org/articles/07901; https://doaj.org/toc/2050-084X |
DOI: | 10.7554/eLife.07901 |
URL الوصول: | https://doaj.org/article/0d0f8d0b5be34f09bf296d7672dad2d5 |
رقم الأكسشن: | edsdoj.0d0f8d0b5be34f09bf296d7672dad2d5 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 2050084X |
---|---|
DOI: | 10.7554/eLife.07901 |