دورية أكاديمية
Protocatechuic acid alleviates polycystic ovary syndrome symptoms in mice by PI3K signaling in granulosa cells to relieve ROS pressure and apoptosis
| العنوان: | Protocatechuic acid alleviates polycystic ovary syndrome symptoms in mice by PI3K signaling in granulosa cells to relieve ROS pressure and apoptosis |
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| المؤلفون: | Feihong Wang, Yanyun Yin, Xiaowei Nie, Yijie Zou, Xingli Tong, Yun Tong, Jing Zhou, Yong Tan |
| المصدر: | Gynecological Endocrinology, Vol 39, Iss 1 (2023) |
| بيانات النشر: | Taylor & Francis Group, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Gynecology and obstetrics LCC:Diseases of the endocrine glands. Clinical endocrinology |
| مصطلحات موضوعية: | Protocatechuic acid, polycystic ovary syndrome, ROS, PI3K signaling, Gynecology and obstetrics, RG1-991, Diseases of the endocrine glands. Clinical endocrinology, RC648-665 |
| الوصف: | Background Polycystic ovary syndrome (PCOS) is a complicated gynecological endocrine disease that occurs in women of childbearing age. Protocatechuic acid is a phenol-rich compound derived from herbs and owns vital functions in numerous diseases. Howbeit, protocatechuic acid’s impact on PCOS is unknown.Methods A combination of in vivo and in vitro models was examined in this study. C57BL/6 mice were injected subcutaneously daily with dehydroepiandrosterone to establish a PCOS mouse model, and protocatechuic acid was intraperitoneally injected into PCOS mice. Granulosa cells of PCOS ovaries were also isolated. The function of protocatechuic acid was appraised using enzyme-linked immunosorbent assay, hematoxylin–eosin staining, reactive oxygen species (ROS) and LC3 levels analysis, flow cytometry, quantitative real-time PCR, and western blot. Meanwhile, the mechanism of protocatechuic acid was assessed with a series of molecular experiments.Results Protocatechuic acid owned no apparent toxic effect on mice. Functionally, protocatechuic acid owned a function of mitigating PCOS in vivo. Meanwhile, protocatechuic acid repressed ROS, autophagy, and apoptosis of PCOS ovarian granulosa cells in vitro. Mechanistically, rescue assays elucidated that the protective function of protocatechuic acid against PCOS was interrelated to the activation of the PI3K/AKT/mTOR axis.Conclusion Protocatechuic acid alleviated PCOS symptoms in mice through PI3K signaling in granulosa cells to reduce ROS levels and apoptosis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 09513590 1473-0766 0951-3590 |
| Relation: | https://doaj.org/toc/0951-3590; https://doaj.org/toc/1473-0766 |
| DOI: | 10.1080/09513590.2023.2228917 |
| URL الوصول: | https://doaj.org/article/0d3c97cb7e1d4377889fed854159fcde |
| رقم الأكسشن: | edsdoj.0d3c97cb7e1d4377889fed854159fcde |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 09513590 14730766 |
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| DOI: | 10.1080/09513590.2023.2228917 |