دورية أكاديمية
A novel D(−)-lactic acid-inducible promoter regulated by the GntR-family protein D-LldR of Pseudomonas fluorescens
| العنوان: | A novel D(−)-lactic acid-inducible promoter regulated by the GntR-family protein D-LldR of Pseudomonas fluorescens |
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| المؤلفون: | Kalpana Singh, Satish Kumar Ainala, Yeonhee Kim, Sunghoon Park |
| المصدر: | Synthetic and Systems Biotechnology, Vol 4, Iss 3, Pp 157-164 (2019) |
| بيانات النشر: | KeAi Communications Co., Ltd., 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Biotechnology LCC:Biology (General) |
| مصطلحات موضوعية: | Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5 |
| الوصف: | Lactic acid has two stereoisomers of D(−)- and L(+)-forms, both of which are important monomers of biodegradable plastic, poly-lactic acid. In this study, a novel d-lactate inducible system was identified in Pseudomonas fluorescens A506, partially characterized and tested as biosensor. The d-lactate catabolic operon (lldP-dld-II) was negatively regulated through the inversely transcribed D-lldR (encoding a GntR-type regulator), where the repression is relieved by addition of d-lactate. The derepression was specific to d-lactate and marginally affected by l-lactate. The D-LldR-responsive operator, showing dyad symmetry and separated by one base, was located between +11 and + 27 from the transcription start site of the lldP-dld-II operon. By site-directed mutagenesis, a motif with a dyad symmetry (AATTGGTAtTACCAATT), present in the upstream region of lldP, was identified as essential for the binding of LldR. d-lactate biosensors were developed by connecting the upregulation by d-lactate to a green fluorescent readout. About ~6.0-fold induction by 100 mM d-lactate was observed compared to l-lactate. Keywords: Lactic acid, Enantiomer, Biosensor, Repression |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-805X 08464391 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405805X19300262; https://doaj.org/toc/2405-805X |
| DOI: | 10.1016/j.synbio.2019.08.004 |
| URL الوصول: | https://doaj.org/article/e0d4f5216c084643918317af2b7dd7c6 |
| رقم الأكسشن: | edsdoj.0d4f5216c084643918317af2b7dd7c6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2405805X 08464391 |
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| DOI: | 10.1016/j.synbio.2019.08.004 |