دورية أكاديمية
Resveratrol inhibits ACHN cells via regulation of histone acetylation
| العنوان: | Resveratrol inhibits ACHN cells via regulation of histone acetylation |
|---|---|
| المؤلفون: | Lili Dai, Lingyan Chen, Wenjing Wang, Peizheng Lin |
| المصدر: | Pharmaceutical Biology, Vol 58, Iss 1, Pp 231-238 (2020) |
| بيانات النشر: | Taylor & Francis Group, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | renal cell carcinoma, mmp-2/-9, proliferation, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Context The relationship between resveratrol and histone acetylation in renal cell carcinoma (RCC) has not yet been reported. Objective To explore the functional role of resveratrol in RCC. Materials and methods Functional experiments were performed to determine proliferatio n of ACHN cells with treatment of resveratrol (0, 7.8125, 15.625, 31.25 and 62.5 μg/mL, for 12, 24 and 48 h of culture) or 0.1 μM SAHA. The enzyme activities of MMP-2/-9 were measured by gelatine zymography and histone acetylation by Western blot. Results When the cells were treated with 15.625, 31.25 and 62.5 μg/mL resveratrol, ACHN cells viability was 73.2 ± 3.5%, 61.4 ± 3.1%, 50.2 ± 4.7% for 12 h, 62.7 ± 4.5%, 52.4 ± 5.5%, 40.2 ± 3.8% for 24 h, and 60.8 ± 3.7%, 39.4 ± 5.1%, 37.6 ± 2.7% for 48 h, and the wound closure (%) of migration was increased from 0.6 to 0.7, 0.85, 0.9 for 12 h and from 0.23 to 0.3, 0.48, 0.59 for 24 h. The invasion rate was 8.5 ± 0.9%, 7.4 ± 0.3% and 5.8 ± 0.6%, and cell cycle was arrested at G1 from 42.5 ± 2.9% to 55.3 ± 5.7%, 59.8 ± 3.4%, 68.7 ± 4.6%. MMP-2/-9 expression (p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1388-0209 1744-5116 13880209 |
| Relation: | https://doaj.org/toc/1388-0209; https://doaj.org/toc/1744-5116 |
| DOI: | 10.1080/13880209.2020.1738503 |
| URL الوصول: | https://doaj.org/article/0d9a2db3851943e5b7e4c781ce886f69 |
| رقم الأكسشن: | edsdoj.0d9a2db3851943e5b7e4c781ce886f69 |
| قاعدة البيانات: | Directory of Open Access Journals |
PDF full text from Publisher 
Full Text Finder | تدمد: | 13880209 17445116 |
|---|---|
| DOI: | 10.1080/13880209.2020.1738503 |