دورية أكاديمية
Frequency determination of breast tumor-reactive CD4 and CD8 T cells in humans: unveiling the antitumor immune response
| العنوان: | Frequency determination of breast tumor-reactive CD4 and CD8 T cells in humans: unveiling the antitumor immune response |
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| المؤلفون: | Mariana Pereira Pinho, Thiago Andrade Patente, Elizabeth Alexandra Flatow, Federica Sallusto, José Alexandre Marzagão Barbuto |
| المصدر: | OncoImmunology, Vol 8, Iss 8 (2019) |
| بيانات النشر: | Taylor & Francis Group, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Immunologic diseases. Allergy LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | cancer immunosurveillance, tumor-specific t lymphocytes, tumor-infiltrating lymphocytes, t cell repertoire, dendritic cell, Immunologic diseases. Allergy, RC581-607, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | As cancer immunotherapy gains importance, the determination of a patient’s ability to react to his/her tumor is unquestionably relevant. Though the presence of T cells that recognize specific tumor antigens is well established, the total frequency of tumor-reactive T cells in humans is difficult to assess, especially due to the lack of broad analysis techniques. Here, we describe a strategy that allows this determination, in both CD4 and CD8 compartments, using T cell proliferation induced by tumor cell-lysate pulsed dendritic cells as the readout. All 12 healthy donor tested had circulating CD4 and CD8 tumor cell-reactive T cells. The detection of these T cells, not only in the naïve but also in the memory compartment, can be seen as an evidence of tumor immunosurveillance in humans. As expected, breast cancer patients had higher frequencies of blood tumor-reactive T cells, but with differences among breast cancer subtypes. Interestingly, the frequency of blood tumor-reactive T cells in patients did not correlate to the frequency of infiltrating tumor-reactive T cells, highlighting the danger of implying a local tumor response from blood obtained data. In conclusion, these data add T cell evidence to immunosurveillance in humans, confirm that immune parameters in blood may be misleading and describe a tool to follow the tumor-specific immune response in patients and, thus, to design better immunotherapeutic approaches. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2162-402X 2162402X |
| Relation: | https://doaj.org/toc/2162-402X |
| DOI: | 10.1080/2162402X.2019.1607674 |
| URL الوصول: | https://doaj.org/article/0dacdb1f02834a77916bffc981aedd11 |
| رقم الأكسشن: | edsdoj.0dacdb1f02834a77916bffc981aedd11 |
| قاعدة البيانات: | Directory of Open Access Journals |
PDF full text from Publisher | تدمد: | 2162402X |
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| DOI: | 10.1080/2162402X.2019.1607674 |