دورية أكاديمية
Recombinant Human Bone Morphogenetic Protein 6 Delivered Within Autologous Blood Coagulum Restores Critical Size Segmental Defects of Ulna in Rabbits
العنوان: | Recombinant Human Bone Morphogenetic Protein 6 Delivered Within Autologous Blood Coagulum Restores Critical Size Segmental Defects of Ulna in Rabbits |
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المؤلفون: | Lovorka Grgurevic, Hermann Oppermann, Marko Pecin, Igor Erjavec, Hrvoje Capak, Martina Pauk, Sven Karlovic, Vera Kufner, Marija Lipar, Jadranka Bubic Spoljar, Tatjana Bordukalo‐Niksic, Drazen Maticic, Mihaela Peric, Reinhard Windhager, T Kuber Sampath, Slobodan Vukicevic |
المصدر: | JBMR Plus, Vol 3, Iss 5, Pp n/a-n/a (2019) |
بيانات النشر: | Oxford University Press, 2019. |
سنة النشر: | 2019 |
المجموعة: | LCC:Orthopedic surgery LCC:Diseases of the musculoskeletal system |
مصطلحات موضوعية: | BONE MORPHOGENETIC PROTEIN 6, AUTOLOGOUS BLOOD COAGULUM CARRIER, CRITICAL SIZE DEFECT, AUTOLOGOUS BONE GRAFT SUBSTITUTE, FRACTURE HEALING, Orthopedic surgery, RD701-811, Diseases of the musculoskeletal system, RC925-935 |
الوصف: | ABSTRACT BMP2 and BMP7, which use bovine Achilles tendon–derived absorbable collagen sponge and bovine bone collagen as scaffold, respectively, have been approved as bone graft substitutes for orthopedic and dental indications. Here, we describe an osteoinductive autologous bone graft substitute (ABGS) that contains recombinant human BMP6 (rhBMP6) dispersed within autologous blood coagulum (ABC) scaffold. The ABGS is created as an injectable or implantable coagulum gel with rhBMP6 binding tightly to plasma proteins within fibrin meshwork, as examined by dot‐blot assays, and is released slowly as an intact protein over 6 to 8 days, as assessed by ELISA. The biological activity of ABGS was examined in vivo in rats (Rattus norvegicus) and rabbits (Oryctolagus cuniculus). In a rat subcutaneous implant assay, ABGS induced endochondral bone formation, as observed by histology and micro‐CT analyses. In the rabbit ulna segmental defect model, a reproducible and robust bone formation with complete bridging and restoration of the defect was observed, which is dose dependent, as determined by radiographs, micro‐CT, and histological analyses. In ABGS, ABC scaffold provides a permissive environment for bone induction and contributes to the use of lower doses of rhBMP6 compared with BMP7 in bovine bone collagen as scaffold. The newly formed bone undergoes remodeling and establishes cortices uniformly that is restricted to implant site by bridging with host bone. In summary, ABC carrier containing rhBMP6 may serve as an osteoinductive autologous bone graft substitute for several orthopedic applications that include delayed and nonunion fractures, anterior and posterior lumbar interbody fusion, trauma, and nonunions associated with neurofibromatosis type I. © 2018 American Society for Bone and Mineral Research. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2473-4039 |
Relation: | https://doaj.org/toc/2473-4039 |
DOI: | 10.1002/jbm4.10085 |
URL الوصول: | https://doaj.org/article/0dfcc4199aff4853bece23bbfcf45680 |
رقم الأكسشن: | edsdoj.0dfcc4199aff4853bece23bbfcf45680 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 24734039 |
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DOI: | 10.1002/jbm4.10085 |