دورية أكاديمية
Cdon ablation in motor neurons causes age‐related motor neuron degeneration and impaired sciatic nerve repair
| العنوان: | Cdon ablation in motor neurons causes age‐related motor neuron degeneration and impaired sciatic nerve repair |
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| المؤلفون: | Sunghee Kim, Subin An, Jinwoo Lee, Yideul Jeong, Chang‐Lim You, Hyebeen Kim, Ju‐Hyeon Bae, Chae‐Eun Yun, Dongryul Ryu, Gyu‐Un Bae, Jong‐Sun Kang |
| المصدر: | Journal of Cachexia, Sarcopenia and Muscle, Vol 14, Iss 5, Pp 2239-2252 (2023) |
| بيانات النشر: | Wiley, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Diseases of the musculoskeletal system LCC:Human anatomy |
| مصطلحات موضوعية: | Amyotrophic lateral sclerosis, Cdon, Motor neuron, Muscle atrophy, Neuromuscular junction, Diseases of the musculoskeletal system, RC925-935, Human anatomy, QM1-695 |
| الوصف: | Abstract Background The functional deterioration and loss of motor neurons are tightly associated with degenerative motor neuron diseases and aging‐related muscle wasting. Motor neuron diseases or aging‐related muscle wasting in turn contribute to increased risk of adverse health outcomes in the elderly. Cdon (cell adhesion molecule‐downregulated oncogene) belongs to the immunoglobulin superfamily of cell adhesion molecule and plays essential roles in multiple signalling pathways, including sonic hedgehog (Shh), netrin, and cadherin‐mediated signalling. Cdon as a Shh coreceptor plays a critical role in motor neuron specification during embryonic development. However, its role in adult motor neuron function is unknown. Methods Hb9‐Cre recombinase‐driven motor neuron‐specific Cdon deficient mice (mnKO) and a compound mutant mice (mnKO::SOD1G93A) were generated to investigate the role of Cdon in motor neuron degeneration. Motor neuron regeneration was examined by using a sciatic nerve crush injury model. To investigate the phenotype, physical activity, compound muscle action potential, immunostaining, and transmission electron microscopy were carried out. In the mechanism study, RNA sequencing and RNA/protein analyses were employed. Results Mice lacking Cdon in motor neurons exhibited middle age onset lethality and aging‐related decline in motor function. In the sciatic nerve crush injury model, mnKO mice exhibited an impairment in motor function recovery evident by prolonged compound muscle action potential duration (4.63 ± 0.35 vs. 3.93 ± 0.22 s for f/f, P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2190-6009 2190-5991 |
| Relation: | https://doaj.org/toc/2190-5991; https://doaj.org/toc/2190-6009 |
| DOI: | 10.1002/jcsm.13308 |
| URL الوصول: | https://doaj.org/article/c0e147277b91460d85118f3f0d19ecb2 |
| رقم الأكسشن: | edsdoj.0e147277b91460d85118f3f0d19ecb2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21906009 21905991 |
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| DOI: | 10.1002/jcsm.13308 |