دورية أكاديمية
Mechanisms of Nucleosome Reorganization by PARP1
| العنوان: | Mechanisms of Nucleosome Reorganization by PARP1 |
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| المؤلفون: | Natalya V. Maluchenko, Dmitry K. Nilov, Sergey V. Pushkarev, Elena Y. Kotova, Nadezhda S. Gerasimova, Mikhail P. Kirpichnikov, Marie-France Langelier, John M. Pascal, Md. Sohail Akhtar, Alexey V. Feofanov, Vasily M. Studitsky |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 22, p 12127 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | nucleosome, poly(ADP-ribose) polymerase 1, spFRET microscopy, molecular dynamics, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Poly(ADP-ribose) polymerase 1 (PARP1) is an enzyme involved in DNA repair, chromatin organization and transcription. During transcription initiation, PARP1 interacts with gene promoters where it binds to nucleosomes, replaces linker histone H1 and participates in gene regulation. However, the mechanisms of PARP1-nucleosome interaction remain unknown. Here, using spFRET microscopy, molecular dynamics and biochemical approaches we identified several different PARP1-nucleosome complexes and two types of PARP1 binding to mononucleosomes: at DNA ends and end-independent. Two or three molecules of PARP1 can bind to a nucleosome depending on the presence of linker DNA and can induce reorganization of the entire nucleosome that is independent of catalytic activity of PARP1. Nucleosome reorganization depends upon binding of PARP1 to nucleosomal DNA, likely near the binding site of linker histone H1. The data suggest that PARP1 can induce the formation of an alternative nucleosome state that is likely involved in gene regulation and DNA repair. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 22221212 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/22/22/12127; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms222212127 |
| URL الوصول: | https://doaj.org/article/0f2f4ab1755440bd8cc0c4ed3e76e9ad |
| رقم الأكسشن: | edsdoj.0f2f4ab1755440bd8cc0c4ed3e76e9ad |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22221212 14220067 16616596 |
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| DOI: | 10.3390/ijms222212127 |