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Cell activation-based screening of natively paired human T cell receptor repertoires

التفاصيل البيبلوغرافية
العنوان: Cell activation-based screening of natively paired human T cell receptor repertoires
المؤلفون: Ahmed S. Fahad, Cheng Yu Chung, Sheila N. López Acevedo, Nicoleen Boyle, Bharat Madan, Matías F. Gutiérrez-González, Rodrigo Matus-Nicodemos, Amy D. Laflin, Rukmini R. Ladi, John Zhou, Jacy Wolfe, Sian Llewellyn-Lacey, Richard A. Koup, Daniel C. Douek, Henry H. Balfour, David A. Price, Brandon J. DeKosky
المصدر: Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
بيانات النشر: Nature Portfolio, 2023.
سنة النشر: 2023
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Abstract Adoptive immune therapies based on the transfer of antigen-specific T cells have been used successfully to treat various cancers and viral infections, but improved techniques are needed to identify optimally protective human T cell receptors (TCRs). Here we present a high-throughput approach to the identification of natively paired human TCRα and TCRβ (TCRα:β) genes encoding heterodimeric TCRs that recognize specific peptide antigens bound to major histocompatibility complex molecules (pMHCs). We first captured and cloned TCRα:β genes from individual cells, ensuring fidelity using a suppression PCR. We then screened TCRα:β libraries expressed in an immortalized cell line using peptide-pulsed antigen-presenting cells and sequenced activated clones to identify the cognate TCRs. Our results validated an experimental pipeline that allows large-scale repertoire datasets to be annotated with functional specificity information, facilitating the discovery of therapeutically relevant TCRs.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
Relation: https://doaj.org/toc/2045-2322
DOI: 10.1038/s41598-023-31858-4
URL الوصول: https://doaj.org/article/eeee0fb53a204636b7baa68d6140de84
رقم الأكسشن: edsdoj.0fb53a204636b7baa68d6140de84
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-023-31858-4