دورية أكاديمية
Engineering a marine microalga Chlorella sp. as the cell factory
| العنوان: | Engineering a marine microalga Chlorella sp. as the cell factory |
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| المؤلفون: | Xinping Gu, Ying Deng, Aoqi Wang, Qinhua Gan, Yi Xin, Kalyanee Paithoonrangsarid, Yandu Lu |
| المصدر: | Biotechnology for Biofuels and Bioproducts, Vol 16, Iss 1, Pp 1-9 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Fuel |
| مصطلحات موضوعية: | Marine microalgae, Chlorella, Transformation, Cell factory, Biotechnology, TP248.13-248.65, Fuel, TP315-360 |
| الوصف: | Abstract The use of marine microalgae in industrial systems is attractive for converting CO2 into value-added products using saline water and sunlight. The plant nature and demonstrated industrial potential facilitate Chlorella spp. as excellent model organisms for both basic research and commercial application. However, the transformation method has not been developed in marine Chlorella spp., thus genetic engineering is hindered in exploiting the industrial potentialities of these strains. In this study, we provided a transformation protocol for the marine Chlorella strain MEM25, which showed robust characteristics, including high production of proteins and polyunsaturated fatty acids in multiple cultivation systems over various spatial–temporal scales. We showed that transformants could be obtained in a dramatically time-saving manner (comparable to Saccharomyces cerevisiae) with four functional proteins expressed properly. The transgenes are integrated into the genome and can be successfully inherited for more than two years. The development of a marine Chlorella transformation method, in combination with the complete genome, will greatly facilitate more comprehensive mechanism studies and provide possibilities to use this species as chassis for synthetic biology to produce value-added compounds with mutual advantage in neutralization of CO2 in commercial scales. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2731-3654 |
| Relation: | https://doaj.org/toc/2731-3654 |
| DOI: | 10.1186/s13068-023-02384-2 |
| URL الوصول: | https://doaj.org/article/0fb8af772c8e43a484107f815004ed59 |
| رقم الأكسشن: | edsdoj.0fb8af772c8e43a484107f815004ed59 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 27313654 |
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| DOI: | 10.1186/s13068-023-02384-2 |