دورية أكاديمية
Study of the correlation between blood cholinesterases activity, urinary dialkyl phosphates, and the frequency of micronucleated polychromatic erythrocytes in rats exposed to disulfoton
| العنوان: | Study of the correlation between blood cholinesterases activity, urinary dialkyl phosphates, and the frequency of micronucleated polychromatic erythrocytes in rats exposed to disulfoton |
|---|---|
| المؤلفون: | Mariane Gonçalves Santos, Ricardo Vilela Vitor, Maurício Gustavo Nakamura, Luana de Souza Morelini, Rafaela Scalco Ferreira, Alexandre Giusti Paiva, Luciana Azevedo, Vanessa Bergamin Boralli Marques, Isarita Martins, Eduardo Costa Figueiredo |
| المصدر: | Brazilian Journal of Pharmaceutical Sciences, Vol 49, Iss 1, Pp 149-154 (2013) |
| بيانات النشر: | Universidade de São Paulo, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Pharmacy and materia medica |
| مصطلحات موضوعية: | Pesticidas, Organofosforados, Dissulfoton, Dialquil fosfatos, Colinesterase sanguínea, Eritrócitos policromáticos micronucleados, Pesticides, Organophosphate, Disulfoton, Dialkyl phosphate, Blood cholinesterase, Micronucleated polychromatic erythrocytes, Pharmacy and materia medica, RS1-441 |
| الوصف: | Organophosphates (OPs) are widely used as pesticides, and its urinary metabolites as well as the blood cholinesterases (ChEs) activity have been reported as possible biomarkers for the assessment of this pesticide exposure. Moreover, the OPs can induce mutagenesis, and the bone marrow micronucleus test is an efficient way to assess this chromosomal damage. This paper reports a study carried out to verify the correlation among the disulfoton exposure, blood ChEs activity, urinary diethyl thiophosphate (DETP), and diethyl dithiophosphate (DEDTP), as well as micronucleated polychromatic erythrocytes (MNPCEs) frequency. Four groups of rats (n=12) were exposed to disulfoton at 0, 2.8, 4.7, and 6.6 mg kg-1 body weight. The blood ChEs activity, urinary DETP and DEDTP concentrations, and MNPCEs frequency were determined. It was observed that the plasmatic and erythrocytary ChEs activity decreased from 2.9% to 0.5% and from 35.9 to 3.3%, respectively, when the disulfoton dose was increased from 0 to 6.6 mg kg-1 (correlation of 0.99). Urinary DETP and DEDTP concentrations, as well as the MNPCEs frequency, increased from 0 to 6.58 µg mL-1, from 0 to 0.04 µg mL-1, and from 0 to 1.4%, respectively, when the disulfoton dose was increased from 0 to 6.58 mg kg-1 body weight.Os organofosforados (OPs) são amplamente usados como praguicidas e a atividade da colinesterase sanguínea bem como os metabólitos urinários desses praguicidas têm sido reportados como biomarcadores eficazes para avaliar casos de exposição. Além disso, os OPs podem induzir mutagênese e o teste de micronúcleo de medula óssea é uma boa alternativa para avaliar os danos cromossômicos. Esse artigo reporta um estudo sobre a correlação entre a exposição a dissulfoton, a atividade da colinesterase sanguínea, a excreção urinária de dietil tiofosfato e dietil ditiofosfato e a frequência de micronúcleos em eritrócitos policromáticos. Quatro grupos de ratos (n=12) foram expostos a dissulfotom nas doses de 0, 2,8, 4,7, e 6,6 mg kg-1 de peso corpóreo. A atividade da colinesterase sanguínea as concentrações urinárias de dietil tiofosfato e dietil ditiofosfato e a frequência de micronúcleos foram determinadas. Os resultados demonstraram que as atividades da colinesterase plasmática e eritrocitária diminuíram de 2,9 para 0,5% e de 35,9 para 3,3% , respectivamente, quando a dose de dissulfoton foi aumentada de 0 para 6,6 mg kg-1 (correlação de 0,99). As concentrações urinárias de dietil tiofosfato e dietil ditiofosfato bem como a frequência de micronúcleos aumentaram de 0 a 6,56 µg mL-1, 0 a 0.04 µg mL-1 e de 0 a 1.4%, respectivamente, quando a dose de dissulfotom foi aumentada de 0 a 6,58 mg kg-1. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1984-8250 2175-9790 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100016; https://doaj.org/toc/1984-8250; https://doaj.org/toc/2175-9790 |
| URL الوصول: | https://doaj.org/article/0fcda4a9be40410496a17637cfb554c2 |
| رقم الأكسشن: | edsdoj.0fcda4a9be40410496a17637cfb554c2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19848250 21759790 |
|---|