دورية أكاديمية
Increased mTOR Signaling and Impaired Autophagic Flux Are Hallmarks of SARS-CoV-2 Infection
| العنوان: | Increased mTOR Signaling and Impaired Autophagic Flux Are Hallmarks of SARS-CoV-2 Infection |
|---|---|
| المؤلفون: | Érika Pereira Zambalde, Thomaz Luscher Dias, Grazielle Celeste Maktura, Mariene R. Amorim, Bianca Brenha, Luana Nunes Santos, Lucas Buscaratti, João Gabriel de Angeli Elston, Mariana Camargo Silva Mancini, Isadora Carolina Betim Pavan, Daniel A. Toledo-Teixeira, Karina Bispo-dos-Santos, Pierina L. Parise, Ana Paula Morelli, Luiz Guilherme Salvino da Silva, Ícaro Maia Santos de Castro, Tatiana D. Saccon, Marcelo A. Mori, Fabiana Granja, Helder I. Nakaya, Jose Luiz Proenca-Modena, Henrique Marques-Souza, Fernando Moreira Simabuco |
| المصدر: | Current Issues in Molecular Biology, Vol 45, Iss 1, Pp 327-336 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | COVID-19, single-cell analysis, autophagic flux, SARS-CoV-2, mTOR, Biology (General), QH301-705.5 |
| الوصف: | The COVID-19 (Coronavirus Disease 2019), caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), severely affects mainly individuals with pre-existing comorbidities. Here our aim was to correlate the mTOR (mammalian/mechanistic Target of Rapamycin) and autophagy pathways with the disease severity. Through western blotting and RNA analysis, we found increased mTOR signaling and suppression of genes related to autophagy, lysosome, and vesicle fusion in Vero E6 cells infected with SARS-CoV-2 as well as in transcriptomic data mining of bronchoalveolar epithelial cells from severe COVID-19 patients. Immunofluorescence co-localization assays also indicated that SARS-CoV-2 colocalizes within autophagosomes but not with a lysosomal marker. Our findings indicate that SARS-CoV-2 can benefit from compromised autophagic flux and inhibited exocytosis in individuals with chronic hyperactivation of mTOR signaling. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1467-3045 1467-3037 |
| Relation: | https://www.mdpi.com/1467-3045/45/1/23; https://doaj.org/toc/1467-3037; https://doaj.org/toc/1467-3045 |
| DOI: | 10.3390/cimb45010023 |
| URL الوصول: | https://doaj.org/article/100e2fe393e1485f9350e2393da3bc1e |
| رقم الأكسشن: | edsdoj.100e2fe393e1485f9350e2393da3bc1e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14673045 14673037 |
|---|---|
| DOI: | 10.3390/cimb45010023 |