دورية أكاديمية
Cell death induced by tamoxifen in human blood lymphocytes cultivated in vitro = Morte celular induzida pelo tamoxifeno em linfócitos humanos cultivados in vitro
| العنوان: | Cell death induced by tamoxifen in human blood lymphocytes cultivated in vitro = Morte celular induzida pelo tamoxifeno em linfócitos humanos cultivados in vitro |
|---|---|
| المؤلفون: | Selma Candelária Genari, Heidi Dolder, Naila Francis Paulo de Oliveira |
| المصدر: | Acta Scientiarum: Biological Sciences, Vol 32, Iss 4, Pp 415-421 (2010) |
| بيانات النشر: | Universidade Estadual de Maringá, 2010. |
| سنة النشر: | 2010 |
| المجموعة: | LCC:Biology (General) LCC:Microbiology |
| مصطلحات موضوعية: | apoptosis, autophagy, chemotherapeutic, cytotoxicity, morphology, ultrastructure, apoptose, autofagia, quimioterápico, citotoxicidade, morfologia, ultraestrutura, Biology (General), QH301-705.5, Microbiology, QR1-502 |
| الوصف: | Many chemotherapeutic agents with a potential against solid tumors or leukemia can cause lymphopenia. Tamoxifen (TAM) is a synthetic non-steroidal anti-estrogen drug employed in female breast cancer treatment. The present study investigated the capacity of TAM to induce cell death in human lymphocytes cultivated in vitro. Lymphocytes were obtained from young (25-30 years; n = 3) and elderly women (58-77 years; n = 3) and cultivated for 24 or 48h, with or without TAM (20 ƒÊM). After the culture, cell viability, immunocytochemical response and ultrastructure were evaluated. TAM affected lymphocytes in a time- dependent manner, and cells obtained from elderly women were the most sensitive to TAM. Immunocytochemicalanalysis evidenced higher frequency of apoptosis in treated cells, and the ultrastructural study revealed autophagic vacuoles, differing from the controls. In summary, the treated lymphocytes were affected by TAM, leading to cell death by apoptosis and autophagy.Muitos agentes quimioterapicos com potencial contra tumores solidos ou leucemias podem causar linfopenia. O Tamoxifeno (TAM) e um agente antiestrogeno nao-esteroidal empregado no tratamento de cancer de mama feminino. O presente trabalho investigou a capacidade do TAM em induzir morte celular em linfocitos humanos cultivados in vitro. Oslinfocitos foram obtidos de mulheres jovens (25-30 anos; n = 3) e idosas (58-77 anos; n = 3) e cultivados por 24 ou 48h, com ou sem TAM (20 ƒÊM). Apos a cultura, foram analisadas a viabilidade celular, a resposta imunocitoquimica e a ultraestrutura. Os resultados indicam que o Tamoxifeno induziu morte celular em linfocitos de ambos os grupos, entretanto, as celulas das mulheres idosas apresentaram-se mais sensiveis ao tratamento. A analise imunocitoquimica mostrou maior frequencia de apoptose nas celulas tratadas e o estudo ultraestrutural revelou vacuolos autofagicos nos linfocitos expostos ao Tamoxifeno. Em conclusao, nosso estudo revelou que o TAM induziu morte celular por apoptose e autofagia. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1679-9283 1807-863X |
| Relation: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7015/7015; https://doaj.org/toc/1679-9283; https://doaj.org/toc/1807-863X |
| URL الوصول: | https://doaj.org/article/1017fea2618d4f679a9f6c68ee7aec40 |
| رقم الأكسشن: | edsdoj.1017fea2618d4f679a9f6c68ee7aec40 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16799283 1807863X |
|---|