دورية أكاديمية
A Putative Apoplastic Effector of Clavibacter capsici, ChpGCc as Hypersensitive Response and Virulence (Hrv) Protein in Plants
| العنوان: | A Putative Apoplastic Effector of Clavibacter capsici, ChpGCc as Hypersensitive Response and Virulence (Hrv) Protein in Plants |
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| المؤلفون: | Eom-Ji Oh, In Sun Hwang, Choon-Tak Kwon, Chang-Sik Oh |
| المصدر: | Molecular Plant-Microbe Interactions, Vol 37, Iss 4, Pp 370-379 (2024) |
| بيانات النشر: | The American Phytopathological Society, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Microbiology LCC:Botany |
| مصطلحات موضوعية: | apoplastic effector, bacterial canker, Clavibacter, Hrv, hypersensitive response, serine protease, Microbiology, QR1-502, Botany, QK1-989 |
| الوصف: | Clavibacter bacteria use secreted apoplastic effectors, such as putative serine proteases, for virulence in host plants and for hypersensitive response (HR) induction in nonhost plants. Previously, we have shown that Clavibacter capsici ChpGCc is important for the necrosis development in pepper (Capsicum annuum) leaves. Here, we determine the function of ChpGCc, along with three paralogous proteins, for HR induction in the apoplastic space of a nonhost plant, Nicotiana tabacum. The full-length and signal peptide–deleted (ΔSP) mature forms of all proteins fused with the tobacco PR1b signal sequence were generated. The full-length and ΔSP forms of ChpGCc and only the ΔSP forms of ChpECc and Pat-1Cc, but none of the ChpCCc, triggered HR. Based on the predicted protein structures, ChpGCc carries amino acids for a catalytic triad and a disulfide bridge in positions like Pat-1Cm. Substituting these amino acids of ChpGCc with alanine abolished or reduced HR-inducing activity. To determine whether these residues are important for necrosis development in pepper, alanine-substituted chpGCc genes were transformed into the C. capsici PF008ΔpCM1 strain, which lacks the intact chpGCc gene. The strain with any variants failed to restore the necrosis-causing ability. These results suggest that ChpGCc has a dual function as a virulence factor in host plants and an HR elicitor in nonhost plants. Based on our findings and previous results, we propose Clavibacter apoplastic effectors, such as ChpGCc, Pat-1Cm, Chp-7Cs, and ChpGCm, as hypersensitive response and virulence (Hrv) proteins that display phenotypic similarities to the hypersensitive response and pathogenicity (Hrp) proteins found in gram-negative bacteria. [Graphic: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1943-7706 0894-0282 |
| Relation: | https://doaj.org/toc/0894-0282; https://doaj.org/toc/1943-7706 |
| DOI: | 10.1094/MPMI-09-23-0145-R |
| URL الوصول: | https://doaj.org/article/c10de74b8ee94cabb1c4187767f1a4fd |
| رقم الأكسشن: | edsdoj.10de74b8ee94cabb1c4187767f1a4fd |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19437706 08940282 |
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| DOI: | 10.1094/MPMI-09-23-0145-R |