دورية أكاديمية
Triage of high-risk HPV-positive women in population-based screening by miRNA expression analysis in cervical scrapes; a feasibility study
| العنوان: | Triage of high-risk HPV-positive women in population-based screening by miRNA expression analysis in cervical scrapes; a feasibility study |
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| المؤلفون: | Iris Babion, Barbara C. Snoek, Putri W. Novianti, Annelieke Jaspers, Nienke van Trommel, Daniëlle A. M. Heideman, Chris J. L. M. Meijer, Peter J. F. Snijders, Renske D. M. Steenbergen, Saskia M. Wilting |
| المصدر: | Clinical Epigenetics, Vol 10, Iss 1, Pp 1-10 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Medicine LCC:Genetics |
| مصطلحات موضوعية: | miRNA, HPV, Cervical cancer, CIN, Scrape, Triage, Medicine, Genetics, QH426-470 |
| الوصف: | Abstract Background Primary testing for high-risk HPV (hrHPV) is increasingly implemented in cervical cancer screening programs. Many hrHPV-positive women, however, harbor clinically irrelevant infections, demanding additional disease markers to prevent over-referral and over-treatment. Most promising biomarkers reflect molecular events relevant to the disease process that can be measured objectively in small amounts of clinical material, such as miRNAs. We previously identified eight miRNAs with altered expression in cervical precancer and cancer due to either methylation-mediated silencing or chromosomal alterations. In this study, we evaluated the clinical value of these eight miRNAs on cervical scrapes to triage hrHPV-positive women in cervical screening. Results Expression levels of the eight candidate miRNAs in cervical tissue samples (n = 58) and hrHPV-positive cervical scrapes from a screening population (n = 187) and cancer patients (n = 38) were verified by quantitative RT-PCR. In tissue samples, all miRNAs were significantly differentially expressed (p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1868-7075 1868-7083 |
| Relation: | http://link.springer.com/article/10.1186/s13148-018-0509-9; https://doaj.org/toc/1868-7075; https://doaj.org/toc/1868-7083 |
| DOI: | 10.1186/s13148-018-0509-9 |
| URL الوصول: | https://doaj.org/article/cd119d29ec0d48f083f968d98b60c086 |
| رقم الأكسشن: | edsdoj.119d29ec0d48f083f968d98b60c086 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 18687075 18687083 |
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| DOI: | 10.1186/s13148-018-0509-9 |