دورية أكاديمية
Differentiation of Pseudomonas syringae Pathovars Originating from Stone Fruits
| العنوان: | Differentiation of Pseudomonas syringae Pathovars Originating from Stone Fruits |
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| المؤلفون: | Katarina Gašić, Milan Ivanović, Nemanja Kuzmanović, Aleksa Obradović |
| المصدر: | Pesticidi i Fitomedicina, Vol 27, Iss 3, Pp 219-229 (2012) |
| بيانات النشر: | Institute of Pesticides and Environmental Protection, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Plant culture |
| مصطلحات موضوعية: | Pseudomonas syringae, Stone fruit, Identification, PCR, Plant culture, SB1-1110 |
| الوصف: | Due to an overlapping host range, similar symptomatology and many common characteristics,Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified.In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae,morsprunorum and persicae, we studied the suitability and differentiating potential ofsome standard bacteriological and molecular methods. Differentiation of the strains wasperformed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growthand utilization of various carbon sources. PCR method enabled the detection of toxin-producinggenes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichumcandidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms.Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods,showed different intensity of reaction of the inoculated material which could separate pv.syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR withREP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Serbian |
| تدمد: | 1820-3949 |
| Relation: | http://www.pesting.org.rs/download.php/documents/27-3/27-3%20219-229.pdf; https://doaj.org/toc/1820-3949 |
| URL الوصول: | https://doaj.org/article/12321279dad14ff48dc0332094650017 |
| رقم الأكسشن: | edsdoj.12321279dad14ff48dc0332094650017 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 18203949 |
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