دورية أكاديمية
Consistent global structures of complex RNA states through multidimensional chemical mapping
| العنوان: | Consistent global structures of complex RNA states through multidimensional chemical mapping |
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| المؤلفون: | Clarence Yu Cheng, Fang-Chieh Chou, Wipapat Kladwang, Siqi Tian, Pablo Cordero, Rhiju Das |
| المصدر: | eLife, Vol 4 (2015) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | non-coding RNA, riboswitch, ribozyme, structure prediction, next-generation sequencing, high-throughput, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Accelerating discoveries of non-coding RNA (ncRNA) in myriad biological processes pose major challenges to structural and functional analysis. Despite progress in secondary structure modeling, high-throughput methods have generally failed to determine ncRNA tertiary structures, even at the 1-nm resolution that enables visualization of how helices and functional motifs are positioned in three dimensions. We report that integrating a new method called MOHCA-seq (Multiplexed •OH Cleavage Analysis with paired-end sequencing) with mutate-and-map secondary structure inference guides Rosetta 3D modeling to consistent 1-nm accuracy for intricately folded ncRNAs with lengths up to 188 nucleotides, including a blind RNA-puzzle challenge, the lariat-capping ribozyme. This multidimensional chemical mapping (MCM) pipeline resolves unexpected tertiary proximities for cyclic-di-GMP, glycine, and adenosylcobalamin riboswitch aptamers without their ligands and a loose structure for the recently discovered human HoxA9D internal ribosome entry site regulon. MCM offers a sequencing-based route to uncovering ncRNA 3D structure, applicable to functionally important but potentially heterogeneous states. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/07600; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.07600 |
| URL الوصول: | https://doaj.org/article/12f42d281a2e440fb88bdf5da8cab631 |
| رقم الأكسشن: | edsdoj.12f42d281a2e440fb88bdf5da8cab631 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.07600 |