دورية أكاديمية
Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ.
| العنوان: | Whole genome re-sequencing to identify suppressor mutations of mutant and foreign Escherichia coli FtsZ. |
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| المؤلفون: | Kiani A J Arkus Gardner, Masaki Osawa, Harold P Erickson |
| المصدر: | PLoS ONE, Vol 12, Iss 4, p e0176643 (2017) |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | FtsZ is an essential protein for bacterial cell division, where it forms the cytoskeletal scaffold and may generate the constriction force. We have found previously that some mutant and foreign FtsZ that do not complement an ftsZ null can function for cell division in E. coli upon acquisition of a suppressor mutation somewhere in the genome. We have now identified, via whole genome re-sequencing, single nucleotide polymorphisms in 11 different suppressor strains. Most of the mutations are in genes of various metabolic pathways, which may modulate cell division indirectly. Mutations in three genes, ispA, accD and nlpI, may be more directly involved in cell division. In addition to the genomic suppressor mutations, we identified intragenic suppressors of three FtsZ point mutants (R174A, E250K and L272V). |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC5405962?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0176643 |
| URL الوصول: | https://doaj.org/article/12f9463bac6a4699a9d66341ab3548e9 |
| رقم الأكسشن: | edsdoj.12f9463bac6a4699a9d66341ab3548e9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0176643 |