دورية أكاديمية
Timeline of changes in spike conformational dynamics in emergent SARS-CoV-2 variants reveal progressive stabilization of trimer stalk with altered NTD dynamics
العنوان: | Timeline of changes in spike conformational dynamics in emergent SARS-CoV-2 variants reveal progressive stabilization of trimer stalk with altered NTD dynamics |
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المؤلفون: | Sean M Braet, Theresa SC Buckley, Varun Venkatakrishnan, Kim-Marie A Dam, Pamela J Bjorkman, Ganesh S Anand |
المصدر: | eLife, Vol 12 (2023) |
بيانات النشر: | eLife Sciences Publications Ltd, 2023. |
سنة النشر: | 2023 |
المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
مصطلحات موضوعية: | mass spectrometry, HDXMS, conformational dynamics, allostery, protein-protein interactions, SARS-CoV-2, Medicine, Science, Biology (General), QH301-705.5 |
الوصف: | SARS-CoV-2 emergent variants are characterized by increased viral fitness and each shows multiple mutations predominantly localized to the spike (S) protein. Here, amide hydrogen/deuterium exchange mass spectrometry has been applied to track changes in S dynamics from multiple SARS-CoV-2 variants. Our results highlight large differences across variants at two loci with impacts on S dynamics and stability. A significant enhancement in stabilization first occurred with the emergence of D614G S followed by smaller, progressive stabilization in subsequent variants. Stabilization preceded altered dynamics in the N-terminal domain, wherein Omicron BA.1 S showed the largest magnitude increases relative to other preceding variants. Changes in stabilization and dynamics resulting from S mutations detail the evolutionary trajectory of S in emerging variants. These carry major implications for SARS-CoV-2 viral fitness and offer new insights into variant-specific therapeutic development. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2050-084X |
Relation: | https://elifesciences.org/articles/82584; https://doaj.org/toc/2050-084X |
DOI: | 10.7554/eLife.82584 |
URL الوصول: | https://doaj.org/article/e132c898884c4543b6bf006d6de5b0b7 |
رقم الأكسشن: | edsdoj.132c898884c4543b6bf006d6de5b0b7 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 2050084X |
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DOI: | 10.7554/eLife.82584 |