دورية أكاديمية
A Multi-Color Flow Cytometric Assay for Quantifying Dinutuximab Binding to Neuroblastoma Cells in Tumor, Bone Marrow, and Blood
| العنوان: | A Multi-Color Flow Cytometric Assay for Quantifying Dinutuximab Binding to Neuroblastoma Cells in Tumor, Bone Marrow, and Blood |
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| المؤلفون: | Michelle E. Keyel, Kathryn L. Furr, Min H. Kang, C. Patrick Reynolds |
| المصدر: | Journal of Clinical Medicine, Vol 12, Iss 19, p 6223 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | neuroblastoma, GD2, dinutuximab, flow cytometry, Medicine |
| الوصف: | GD2, a disialoganglioside, is present on the surface of most neuroblastomas, as well as on some other cancers, such as melanoma and osteogenic sarcoma. The anti-GD2 antibody ch14.18 (dinutuximab) has an FDA-registered indication for use as maintenance therapy for high-risk neuroblastoma with cytokines and 13-cis-retinoic acid after myeloablative therapy. Recent studies using immunohistochemistry of tumor or tumor cells in marrow have shown that some neuroblastomas are negative for GD2. Dinutuximab and other anti-GD2 antibodies are increasingly used in combination with cytotoxic chemotherapy for treating relapsed neuroblastoma, so it is important to be able to identify patients with tumor cells with low GD2 expression, as such patients may experience toxicity but not benefit from the antibody therapy. As the most common clinical samples available for relapsed neuroblastoma are bone marrow aspirates, we developed a method to quantify dinutuximab binding density and the frequency of neuroblastoma cells positive for the antibody in bone marrow aspirates. Here, we describe a multi-color flow cytometry assay that employs non-GD2 antibodies to identify neuroblastoma cells in a mixed population (tumor, bone marrow, or blood) and an anti-GD2 antibody to quantify both the frequency and density of GD2 expression on neuroblastoma cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 12196223 2077-0383 |
| Relation: | https://www.mdpi.com/2077-0383/12/19/6223; https://doaj.org/toc/2077-0383 |
| DOI: | 10.3390/jcm12196223 |
| URL الوصول: | https://doaj.org/article/1358ef7bbe0243ea9b4f0cee0dc0d359 |
| رقم الأكسشن: | edsdoj.1358ef7bbe0243ea9b4f0cee0dc0d359 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12196223 20770383 |
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| DOI: | 10.3390/jcm12196223 |