دورية أكاديمية
Comparative In Silico Analysis and Functional Characterization of TANK-Binding Kinase 1–Binding Protein 1
| العنوان: | Comparative In Silico Analysis and Functional Characterization of TANK-Binding Kinase 1–Binding Protein 1 |
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| المؤلفون: | Humaira Aziz Sawal, Shagufta Nighat, Tanzeela Safdar, Laiba Anees |
| المصدر: | Bioinformatics and Biology Insights, Vol 17 (2023) |
| بيانات النشر: | SAGE Publishing, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | Protein modelling plays a vital role in the drug discovery process. TANK-binding kinase 1–binding protein 1 is also called an adapter protein, which is encoded by gene TBK1 present in Homo sapiens. It is found in lungs, small intestine, leukocytes, heart, placenta, muscle, kidney, lower level of thymus, and brain. It has a number of protein-binding sites, to which TBK1 and IKBKE bind and perform different functions as immunomodulatory, antiproliferative, and antiviral innate immunity which release different types of interferons. Our study predicts the comparative model of 3-dimensional (3D) structure through different bioinformatics tools that will be helpful for further studies in future. The reactivity and stability of these proteins were evaluated physicochemically and through domain determination and prediction of secondary structure using bioinformatics methods such as ProtParam, Pfam, and SOPMA, respectively. Robetta, an ab initio approach, I-TASSER, and AlphaFold was used for 3D structure prediction, and the models were validated using the SAVESv6.0 (PROCHECK) server. Conclusively, the best 3D structure of TBK1-binding protein 1 was predicted using Robetta software. After unveiling the 3D structure of the novel protein, we concluded that this structure will help us to find out its role other than in antiviral innate immunity and by producing torsion in its 3D structure researchers will be able to detect either this protein is involved in any disease or not because according to previous studies it was not associated with any disease. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1177-9322 11779322 |
| Relation: | https://doaj.org/toc/1177-9322 |
| DOI: | 10.1177/11779322231164828 |
| URL الوصول: | https://doaj.org/article/e13a4ff8ef7f4d1fac688bb7c2a18002 |
| رقم الأكسشن: | edsdoj.13a4ff8ef7f4d1fac688bb7c2a18002 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 11779322 |
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| DOI: | 10.1177/11779322231164828 |