دورية أكاديمية
Human acellular amniotic membrane incorporating exosomes from adipose-derived mesenchymal stem cells promotes diabetic wound healing
العنوان: | Human acellular amniotic membrane incorporating exosomes from adipose-derived mesenchymal stem cells promotes diabetic wound healing |
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المؤلفون: | Shune Xiao, Chunfang Xiao, Yong Miao, Jin Wang, Ruosi Chen, Zhexiang Fan, Zhiqi Hu |
المصدر: | Stem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-16 (2021) |
بيانات النشر: | BMC, 2021. |
سنة النشر: | 2021 |
المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
مصطلحات موضوعية: | Adipose-derived mesenchymal stem cells, Exosomes, Diabetic wound healing, Acellular amniotic membrane, Medicine (General), R5-920, Biochemistry, QD415-436 |
الوصف: | Abstract Background Diabetic wounds threaten the health and quality of life of patients and their treatment remains challenging. ADSC-derived exosomes have shown encouraging results in enhancing diabetic wound healing. However, how to use exosomes in wound treatment effectively is a problem that needs to be addressed at present. Methods A diabetic mouse skin wound model was established. ADSC-derived exosomes (ADSC-Exos) were isolated, and in vitro application of exosomes was evaluated using human umbilical vein endothelial cells (HUVECs) and human dermal fibroblasts (HDFs). After preparation and characterization of a scaffold of human acellular amniotic membrane (hAAM) loaded with ADSC-Exos in vitro, they were transplanted into wounds in vivo and wound healing phenomena were observed by histological and immunohistochemical analyses to identify the wound healing mechanism of the exosome-hAAM composites. Results The hAAM scaffold dressing was very suitable for the delivery of exosomes. ADSC-Exos enhanced the proliferation and migration of HDFs and promoted proliferation and tube formation of HUVECs in vitro. In vivo results from a diabetic skin wound model showed that the hAAM-Exos dressing accelerated wound healing by regulating inflammation, stimulating vascularization, and promoting the production of extracellular matrix. Conclusion Exosome-incorporated hAAM scaffolds showed great potential in promoting diabetic skin wound healing, while also providing strong evidence for the future clinical applications of ADSC-derived exosomes. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1757-6512 |
Relation: | https://doaj.org/toc/1757-6512 |
DOI: | 10.1186/s13287-021-02333-6 |
URL الوصول: | https://doaj.org/article/cec1520286354b5dbb450bf08a6b9223 |
رقم الأكسشن: | edsdoj.1520286354b5dbb450bf08a6b9223 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 17576512 |
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DOI: | 10.1186/s13287-021-02333-6 |