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Modulatory effect of linoleic and oleic acid on cell proliferation and lipid metabolism gene expressions in primary bovine satellite cells

التفاصيل البيبلوغرافية
العنوان: Modulatory effect of linoleic and oleic acid on cell proliferation and lipid metabolism gene expressions in primary bovine satellite cells
المؤلفون: Shah Ahmed Belal, Allur Subramaniyan Sivakumar, Da Rae Kang, Sangbuem Cho, Ho Sung Choe, Kwan Seob Shim
المصدر: Animal Cells and Systems, Vol 22, Iss 5, Pp 324-333 (2018)
بيانات النشر: Taylor & Francis Group, 2018.
سنة النشر: 2018
المجموعة: LCC:Medicine (General)
LCC:Biology (General)
مصطلحات موضوعية: Bovine satellite cells, cell proliferation, gene expression, linoleic acid, oleic acid, Medicine (General), R5-920, Biology (General), QH301-705.5
الوصف: This study was performed to elucidate the effects of linoleic acid (LA), oleic acid (OA) and their combination (LA + OA) on cell proliferation, apoptosis, necrosis, and the lipid metabolism related gene expression in bovine satellite cells (BSCs), isolated from bovine muscles. Cell viability was significantly increased with the OA and LA treatment. Furthermore, LA + OA enhanced cell proliferation in a dose-dependent manner (10 to 100 µM), whereas it lowered at 250 µM. In addition, a cell-cycle analysis showed that 100 µM of LA and OA markedly decreased the G0/G1 phase proportion (62.58% and 61.33%, respectively), compared to controls (68.02%), whereas the S-phase cells’ proportion was increased. The ratio of G2/M phase cells was not significantly different among the groups. Moreover, analyses with AO/EtBr staining showed that no apoptosis occurred. Necrosis were determined by flow cytometry using Annexin V-FITC/PI staining which revealed no early apoptosis in the cells pretreated with LA or OA, but occurred in the LA + OA group. We also analyzed the mRNA expression of lipid metabolizing genes such as peroxisome proliferator receptor alfa (PPARα), peroxisome proliferator receptor gamma (PPARγ), acyl-CoA oxidase (ACOX), lipoprotein lipase (LPL), carnitine palmitoyl transferase (CPT-1), and fatty-acid binding protein4 (FABP4), which were upregulated in LA or OA treated cells compared to the control group. In essence, LA and OA alone promote the cell proliferation without any apoptosis and necrosis, which might upregulate the lipid metabolism related gene expressions, and increase fatty-acid oxidation in the BSCs’ lipid metabolism.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1976-8354
2151-2485
19768354
Relation: https://doaj.org/toc/1976-8354; https://doaj.org/toc/2151-2485
DOI: 10.1080/19768354.2018.1517824
URL الوصول: https://doaj.org/article/a167e06ac16245419f934d3971879944
رقم الأكسشن: edsdoj.167e06ac16245419f934d3971879944
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19768354
21512485
DOI:10.1080/19768354.2018.1517824