دورية أكاديمية
IL-25 Impact on Malignant B Cells Survival and T Cells Activation in Chronic Lymphocytic Leukemia
العنوان: | IL-25 Impact on Malignant B Cells Survival and T Cells Activation in Chronic Lymphocytic Leukemia |
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المؤلفون: | Mehrnoosh Pashei, Farahnaz Ghahremanfard, Ehsan Manouchehri Doulabi, Maral Hemmati, Fatemeh Pak, Parviz Kokhaei |
المصدر: | Iranian Journal of Allergy, Asthma and Immunology, Vol 22, Iss 3 (2023) |
بيانات النشر: | Tehran University of Medical Sciences, 2023. |
سنة النشر: | 2023 |
المجموعة: | LCC:Medicine |
مصطلحات موضوعية: | Chronic lymphocytic leukemia, Interleukin-25, Tumor microenvironment, T helper 2 cells, Medicine |
الوصف: | T cell dysregulation and shift to T helper 2 responses, boosting tumor microenvironment support, contributes to the survival of leukemic B cells in Chronic Lymphocytic Leukemia. Interleukin (IL)-25 is involved in the initiation of T helper 2 cell responses. Signal transduction of IL-25 begins with the heterodimer receptor (IL-17RA/IL-17RB). The presence of IL-25 in the tumor microenvironment may affect the supportive effects of T cells in the surrounding tumor cell environment. The purpose of this study was to evaluate the role of IL-25 in the biology of CLL. IL-17RB expression in CD3+ and CD19+ cells was assessed in isolated peripheral blood mononuclear cells (PBMCs) of nine CLL patients and nine healthy subjects by real-time polymerase chain reaction and flow cytometry. B cells were positively enriched from PBMCs using magnetic-activated cell sorting (MACS). PBMCs and purified leukemic B cells were cultured with recombinant human IL-25 (20ng/ml) for 72 hours, then the viability and apoptosis of cultured cells were measured by MTT assay and AnnexinV/7AAD. Furthermore, the levels of CD69 expression on T lymphocytes and IL-17RB in T and B cells were determined by flow cytometry. The basal level of IL-17RB expression in CLL patients was significantly higher than that in control individuals. In addition, the percentage of IL-17RB+/CD3+, IL-17RB+/CD19+ cells and CD69+/CD3+ cells increased after 72 hours of culture with IL-25 in CLL patients compared to healthy subjects. IL-25 also reduces the apoptosis rate of tumor cells. We found that IL-25 could stimulate T cells in CLL patients and lower B cell death. This suggests that IL-25 might have a role in enhancing the survival of tumor cell by expressing receptors for inflammation, such as IL-17RB, and might be involved in the development of CLL. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1735-1502 1735-5249 |
Relation: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/3676; https://doaj.org/toc/1735-1502; https://doaj.org/toc/1735-5249 |
DOI: | 10.18502/ijaai.v22i3.13058 |
URL الوصول: | https://doaj.org/article/c168465c2be74af4831e847f436fb12d |
رقم الأكسشن: | edsdoj.168465c2be74af4831e847f436fb12d |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 17351502 17355249 |
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DOI: | 10.18502/ijaai.v22i3.13058 |