دورية أكاديمية
Real-time monitoring of T-cell-secreted interferon-γ for the diagnosis of tuberculosis
| العنوان: | Real-time monitoring of T-cell-secreted interferon-γ for the diagnosis of tuberculosis |
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| المؤلفون: | Changlin Wu, Jianan He, Boan Li, YunQing Xu, Dayong Gu, Houming Liu, Dan Zhao, Chaopeng Shao |
| المصدر: | Biotechnology & Biotechnological Equipment, Vol 32, Iss 4, Pp 993-998 (2018) |
| بيانات النشر: | Taylor & Francis Group, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Biotechnology |
| مصطلحات موضوعية: | Surface plasmon resonance, protein microarray, tubercle bacillus, CD4+ T-cells, IFN-γ, label-free, Biotechnology, TP248.13-248.65 |
| الوصف: | In this paper, we report the development of a label-free biosensor, based on surface plasmon resonance, for real-time monitoring of captured human CD4+T-cells, and their dynamic cytokine production upon specific antigen stimulation. Microarrays of CD4+T-cells, and interferon gamma (IFN-γ) specific antibody (Ab) spots were printed side by side onto the poly(OEGMA-co-HEMA) matrix. The placement of CD4+ T-cells near the anti IFN-γ Ab-coated spots ensured a high local concentration of secreted IFN-γ for detection. We have demonstrated that this approach enables the detection of tuberculosis (TB) infection in clinical samples, with an overall sensitivity of 85.5% and an overall specificity of 97.7%. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1310-2818 1314-3530 13102818 |
| Relation: | https://doaj.org/toc/1310-2818; https://doaj.org/toc/1314-3530 |
| DOI: | 10.1080/13102818.2018.1432416 |
| URL الوصول: | https://doaj.org/article/16ace3ebad1544858fde49eeff461f57 |
| رقم الأكسشن: | edsdoj.16ace3ebad1544858fde49eeff461f57 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 13102818 13143530 |
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| DOI: | 10.1080/13102818.2018.1432416 |