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Single-cell RNA-sequence analysis of human bone marrow reveals new targets for isolation of skeletal stem cells using spherical nucleic acids

التفاصيل البيبلوغرافية
العنوان: Single-cell RNA-sequence analysis of human bone marrow reveals new targets for isolation of skeletal stem cells using spherical nucleic acids
المؤلفون: Elloise Z Matthews, Stuart Lanham, Kate White, Maria-Eleni Kyriazi, Konstantina Alexaki, Afaf H El-Sagheer, Tom Brown, Antonios G Kanaras, Jonathan J West, Ben D MacArthur, Patrick S Stumpf, Richard OC Oreffo
المصدر: Journal of Tissue Engineering, Vol 14 (2023)
بيانات النشر: SAGE Publishing, 2023.
سنة النشر: 2023
المجموعة: LCC:Biochemistry
مصطلحات موضوعية: Biochemistry, QD415-436
الوصف: There is a wealth of data indicating human bone marrow contains skeletal stem cells (SSC) with the capacity for osteogenic, chondrogenic and adipogenic differentiation. However, current methods to isolate SSCs are restricted by the lack of a defined marker, limiting understanding of SSC fate, immunophenotype, function and clinical application. The current study applied single-cell RNA-sequencing to profile human adult bone marrow populations from 11 donors and identified novel targets for SSC enrichment. Spherical nucleic acids were used to detect these mRNA targets in SSCs. This methodology was able to rapidly isolate potential SSCs found at a frequency of
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2041-7314
20417314
Relation: https://doaj.org/toc/2041-7314
DOI: 10.1177/20417314231169375
URL الوصول: https://doaj.org/article/aa16f755c38b439d9985a76d8ea427d6
رقم الأكسشن: edsdoj.16f755c38b439d9985a76d8ea427d6
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20417314
DOI:10.1177/20417314231169375