دورية أكاديمية
Preparation of Plant-derived Smoke for Stimulating Seed Germination and Quantification of Karrikins Using High Performance Liquid Chromatography
العنوان: | Preparation of Plant-derived Smoke for Stimulating Seed Germination and Quantification of Karrikins Using High Performance Liquid Chromatography |
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المؤلفون: | Cüneyt Akı, Yasemin Kemeç Hürkan |
المصدر: | Journal of Agricultural Sciences, Vol 29, Iss 3, Pp 800-810 (2023) |
بيانات النشر: | Faculty of Agriculture, Ankara University, 2023. |
سنة النشر: | 2023 |
المجموعة: | LCC:Agriculture (General) |
مصطلحات موضوعية: | smoke water, kar1, karrikinolide, prunus armeniaca, plant growth regulator, Agriculture (General), S1-972 |
الوصف: | Smoke water (SW) is produced naturally or artificially from burning plant material. It provides the germination of the seeds of many plants and accelerates the growth and development of the plant and is also used in many fields of plant science. SW preparation is a relatively easy and inexpensive method, but a standard method for its preparation has not been developed yet. Therefore, the aim of this research is to develop a low-cost efficient method to produce SW, to standardize it and to measure the amount of the main active biomolecule karrikin (KAR1) by HPLC. We also aimed to test and compare the best working concentration of SW and commercially available KAR1 on apricot (Prunus armeniaca L.) seeds. The SWs were diluted to 1:100, 1:500, 1:1000, 1:5000 and 1:10000 ratios, and KAR1 to 0.01 μM, 0.1 μM, 1 μM, 5 μM and 10 μM concentrations. In terms of germination, it was determined that the use of 1:1000 (60%) concentration in the SW group and 1 μM (72%) concentration in the KAR1 group was appropriate. This is the first research in which a standard method was developed for obtaining SW. We think this study will be a guide to researchers who plan to study with smoke water, since we obtained the most concentrated KAR1 according to the literature. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1300-7580 2148-9297 |
Relation: | https://dergipark.org.tr/tr/download/article-file/2710165; https://doaj.org/toc/1300-7580; https://doaj.org/toc/2148-9297 |
DOI: | 10.15832/ankutbd.1189515 |
URL الوصول: | https://doaj.org/article/1701aaafdc624b70bd472b6ab88298f0 |
رقم الأكسشن: | edsdoj.1701aaafdc624b70bd472b6ab88298f0 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 13007580 21489297 |
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DOI: | 10.15832/ankutbd.1189515 |