دورية أكاديمية
أعرض في EDS

Nuclear Factor Kappa-B Protein Levels in Sperm of Obese Men with and without Diabetes; Cellular Approach in Male Infertility

التفاصيل البيبلوغرافية
العنوان: Nuclear Factor Kappa-B Protein Levels in Sperm of Obese Men with and without Diabetes; Cellular Approach in Male Infertility
المؤلفون: Shima Abbasihormozi, Azam Kouhkan, Abdolhossein Shaverdi, Mohammad Ali Sadighi Gilani, Vahab Babapour, Amir Niasari Naslji, Vahid Akbarinehad, AliReza Alizadeh
المصدر: Cell Journal, Vol 25, Iss 1, Pp 17-24 (2023)
بيانات النشر: Royan Institute (ACECR), Tehran, 2023.
سنة النشر: 2023
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: diabetes mellitus, nuclear factor kappa-b, obesity morbid, spermatozoa, type ii, Medicine, Science
الوصف: Objective: Although the role of obesity and diabetes mellitus (DM) in male infertility is well established, little informationabout the underlying cellular mechanisms in infertility is available. In this sense, nuclear factor kappa-B (NF-kB) hasbeen recognized as an important regulator in obesity and DM; However, its function in the pathogenesis of maleinfertility has never been studied in obese or men who suffer from diabetes. Therefore, the main goal of current researchis assessing NF-kB existence and activity in ejaculated human spermatozoa considering the obesity and diabeticscondition of males. Materials and Methods: In an experimental study, the ELISA technique was applied to analyze NF-kB levels in spermof four experimental groups: non-obese none-diabetic men (body mass index (BMI) 30 kg/m2; OB group; n=30), non-obese diabetic men (BMI 30 kg/m2; OB-DM group; n=30) who were presented to Royan Institute Infertility Center.In addition, protein localization was shown by Immunocytofluorescent assay. Sperm features were also evaluated usingCASA. Results: The diabetic men were older than non-diabetic men regardless of obesity status (P=0.0002). Sperm progressivemotility was affected by obesity (P=0.035) and type A sperm progressive motility was affected by DM (P=0.034). Theconcentration of sperm (P=0.013), motility (P=0.025) and morphology (P
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2228-5806
2228-5814
Relation: https://www.celljournal.org/article_696708_aa8e5ce59c9eb7df4a9c905b6de739d2.pdf; https://doaj.org/toc/2228-5806; https://doaj.org/toc/2228-5814
DOI: 10.22074/cellj.2022.557547.1065
URL الوصول: https://doaj.org/article/a18bff4ac1224a33bcf69c80e352cbde
رقم الأكسشن: edsdoj.18bff4ac1224a33bcf69c80e352cbde
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:22285806
22285814
DOI:10.22074/cellj.2022.557547.1065