دورية أكاديمية
Beneficial Effects of Melatonin Combined with Exercise on Endogenous Neural Stem/Progenitor Cells Proliferation after Spinal Cord Injury
| العنوان: | Beneficial Effects of Melatonin Combined with Exercise on Endogenous Neural Stem/Progenitor Cells Proliferation after Spinal Cord Injury |
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| المؤلفون: | Youngjeon Lee, Seunghoon Lee, Sang-Rae Lee, Kanghui Park, Yunkyung Hong, Minkyung Lee, Sookyoung Park, Yunho Jin, Kyu-Tae Chang, Yonggeun Hong |
| المصدر: | International Journal of Molecular Sciences, Vol 15, Iss 2, Pp 2207-2222 (2014) |
| بيانات النشر: | MDPI AG, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | spinal cord injury, melatonin, functional recovery, endogenous neural stem/progenitor, therapeutic exercise, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Endogenous neural stem/progenitor cells (eNSPCs) proliferate and differentiate into neurons and glial cells after spinal cord injury (SCI). We have previously shown that melatonin (MT) plus exercise (Ex) had a synergistic effect on functional recovery after SCI. Thus, we hypothesized that combined therapy including melatonin and exercise might exert a beneficial effect on eNSPCs after SCI. Melatonin was administered twice a day and exercise was performed on a treadmill for 15 min, six days per week for 3 weeks after SCI. Immunohistochemistry and RT-PCR analysis were used to determine cell population for late response, in conjunction with histological examination and motor function test. There was marked improvement in hindlimb function in SCI+MT+Ex group at day 14 and 21 after injury, as documented by the reduced size of the spinal lesion and a higher density of dendritic spines and axons; such functional improvements were associated with increased numbers of BrdU-positive cells. Furthermore, MAP2 was increased in the injured thoracic segment, while GFAP was increased in the cervical segment, along with elevated numbers of BrdU-positive nestin-expressing eNSPCs in the SCI+MT+Ex group. The dendritic spine density was augmented markedly in SCI+MT and SCI+MT+Ex groups.These results suggest a synergistic effect of SCI+MT+Ex might create a microenvironment to facilitate proliferation of eNSPCs to effectively replace injured cells and to improve regeneration in SCI. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 |
| Relation: | http://www.mdpi.com/1422-0067/15/2/2207; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms15022207 |
| URL الوصول: | https://doaj.org/article/18f28b786bed4d39841c6d3fa8cbb6a2 |
| رقم الأكسشن: | edsdoj.18f28b786bed4d39841c6d3fa8cbb6a2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms15022207 |