دورية أكاديمية
Mechanism of Killing Effect of Thioridazine on Human Lung Cancer PC9 Cells
| العنوان: | Mechanism of Killing Effect of Thioridazine on Human Lung Cancer PC9 Cells |
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| المؤلفون: | Li GONG, Yi WANG, Sihao TONG, Liu LIU, Ling NIU, Yuan YUAN, Yangyi BAO |
| المصدر: | Chinese Journal of Lung Cancer, Vol 18, Iss 12, Pp page-page (2015) |
| بيانات النشر: | Chinese Anti-Cancer Association; Chinese Antituberculosis Association, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | Lung cancer, Thioridazine, Apoptosis, Caspase apoptotic pathway, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Background and objective Recent research shows thioridazine which is a kind of phenothiazine antipsychotic drugs can inhibit the proliferation of various tumor cells in vitro, but the role of thioridazine on lung cancer has not been reported. So we choose PC9 cell lines as the research object, the aim is to oberve the killing effect of thioridazine on PC9 cells and investigate its possible mechanism. Methods After being treated with different concentrations of thioridazine, the proliferation of PC9 cells was determined by methyl thiazolyltetrazolium (MTT) assay. Flow cytometry was used to measure the cell cycle distribution and apoptosis. The expressions of cell cycle-associated protein CyclinD1 and apoptosis-related proteins Caspase-3, Caspase-8, Caspase-9, Bcl-2, Bax and Bcl-xl were detected by Western blot. Results The proliferation of PC9 cells was significantly inhibited by thioridazine in a dose- and time-dependent manner. Flow cytometry showed that cell cycle was arrested in G0/G1 phase and the apoptotic rates were significantly increased with the increasing concentration of thioridazine. Compared with the control group, the differences were statistically significant (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Chinese |
| تدمد: | 1009-3419 1999-6187 |
| Relation: | https://doaj.org/toc/1009-3419; https://doaj.org/toc/1999-6187 |
| DOI: | 10.3779/j.issn.1009-3419.2015.12.03 |
| URL الوصول: | https://doaj.org/article/19b856e9633243c9b78a231c493ad2db |
| رقم الأكسشن: | edsdoj.19b856e9633243c9b78a231c493ad2db |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10093419 19996187 |
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| DOI: | 10.3779/j.issn.1009-3419.2015.12.03 |