دورية أكاديمية
A bacteriophage tubulin harnesses dynamic instability to center DNA in infected cells
| العنوان: | A bacteriophage tubulin harnesses dynamic instability to center DNA in infected cells |
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| المؤلفون: | Marcella L Erb, James A Kraemer, Joanna K C Coker, Vorrapon Chaikeeratisak, Poochit Nonejuie, David A Agard, Joe Pogliano |
| المصدر: | eLife, Vol 3 (2014) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | tubulin, cytoskeleton, dynamic instability, spindle, pseudomonas, bacteriophage, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Dynamic instability, polarity, and spatiotemporal organization are hallmarks of the microtubule cytoskeleton that allow formation of complex structures such as the eukaryotic spindle. No similar structure has been identified in prokaryotes. The bacteriophage-encoded tubulin PhuZ is required to position DNA at mid-cell, without which infectivity is compromised. Here, we show that PhuZ filaments, like microtubules, stochastically switch from growing in a distinctly polar manner to catastrophic depolymerization (dynamic instability) both in vitro and in vivo. One end of each PhuZ filament is stably anchored near the cell pole to form a spindle-like array that orients the growing ends toward the phage nucleoid so as to position it near mid-cell. Our results demonstrate how a bacteriophage can harness the properties of a tubulin-like cytoskeleton for efficient propagation. This represents the first identification of a prokaryotic tubulin with the dynamic instability of microtubules and the ability to form a simplified bipolar spindle. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/03197; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.03197 |
| URL الوصول: | https://doaj.org/article/19b8e4b5d4864b308caf5207a33ad3af |
| رقم الأكسشن: | edsdoj.19b8e4b5d4864b308caf5207a33ad3af |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.03197 |