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Low-dose levodopa protects nerve cells from oxidative stress and up-regulates expression of pCREB and CD39.

التفاصيل البيبلوغرافية
العنوان: Low-dose levodopa protects nerve cells from oxidative stress and up-regulates expression of pCREB and CD39.
المؤلفون: Shi-Ying Zhong, Yong-Xing Chen, Min Fang, Xiao-Long Zhu, Yan-Xin Zhao, Xue-Yuan Liu
المصدر: PLoS ONE, Vol 9, Iss 4, p e95387 (2014)
بيانات النشر: Public Library of Science (PLoS), 2014.
سنة النشر: 2014
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: OBJECTIVE: This study aimed to investigate the influence of low-dose levodopa (L-DOPA) on neuronal cell death under oxidative stress. METHODS: PC12 cells were treated with L-DOPA at different concentrations. We detected the L-DOPA induced reactive oxygen species (ROS). Meanwhile, MTT and LDH assay were performed to determine the proliferation and growth of PC12 cells with or without ROS scavenger. In addition, after pretreatment with L-DOPA at different concentrations alone or in combination with CD39 inhibitor, PC12 cells were incubated with hydrogen peroxide (H2O2) and the cell viability was evaluated by MTT and LDH assay. In addition, the expression of pCREB and CD39 was detected by immunofluorescence staining and Western blot assay in both cells and rat's brain after L-DOPA treatment. RESULTS: After treatment with L-DOPA for 3 days, the cell proliferation and growth were promoted when the L-DOPA concentration was 30 µM. Low dose L-DOPA could protect the PC12 cells from H2O2 induced oxidative stress, which was compromised by CD39 inhibitor. In addition, the expression of CD39 and pCREB increased in both PC12 cells and rats' brain after L-DOPA treatment. CONCLUSIONS: L-DOPA at different concentrations has distinct influence on proliferation and growth of PC12 cells, and low dose (
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3990701?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0095387
URL الوصول: https://doaj.org/article/19c3a2a54a404e89ae4ac19f3dbc4bef
رقم الأكسشن: edsdoj.19c3a2a54a404e89ae4ac19f3dbc4bef
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0095387