دورية أكاديمية
ROP18 is a rhoptry kinase controlling the intracellular proliferation of Toxoplasma gondii.
| العنوان: | ROP18 is a rhoptry kinase controlling the intracellular proliferation of Toxoplasma gondii. |
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| المؤلفون: | Hiba El Hajj, Maryse Lebrun, Stefan T Arold, Henri Vial, Gilles Labesse, Jean François Dubremetz |
| المصدر: | PLoS Pathogens, Vol 3, Iss 2, p e14 (2007) |
| بيانات النشر: | Public Library of Science (PLoS), 2007. |
| سنة النشر: | 2007 |
| المجموعة: | LCC:Immunologic diseases. Allergy LCC:Biology (General) |
| مصطلحات موضوعية: | Immunologic diseases. Allergy, RC581-607, Biology (General), QH301-705.5 |
| الوصف: | Toxoplasma gondii is an obligate intracellular parasite for which the discharge of apical organelles named rhoptries is a key event in host cell invasion. Among rhoptry proteins, ROP2, which is the prototype of a large protein family, is translocated in the parasitophorous vacuole membrane during invasion. The ROP2 family members are related to protein-kinases, but only some of them are predicted to be catalytically active, and none of the latter has been characterized so far. We show here that ROP18, a member of the ROP2 family, is located in the rhoptries and re-localises at the parasitophorous vacuole membrane during invasion. We demonstrate that a recombinant ROP18 catalytic domain (amino acids 243-539) possesses a protein-kinase activity and phosphorylate parasitic substrates, especially a 70-kDa protein of tachyzoites. Furthermore, we show that overexpression of ROP18 in transgenic parasites causes a dramatic increase in intra-vacuolar parasite multiplication rate, which is correlated with kinase activity. Therefore, we demonstrate, to our knowledge for the first time, that rhoptries can discharge active protein-kinases upon host cell invasion, which can exert a long-lasting effect on intracellular parasite development and virulence. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1553-7366 1553-7374 |
| Relation: | http://europepmc.org/articles/PMC1797617?pdf=render; https://doaj.org/toc/1553-7366; https://doaj.org/toc/1553-7374 |
| DOI: | 10.1371/journal.ppat.0030014 |
| URL الوصول: | https://doaj.org/article/1a4ed836cb914525abf6a684683e8d55 |
| رقم الأكسشن: | edsdoj.1a4ed836cb914525abf6a684683e8d55 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15537366 15537374 |
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| DOI: | 10.1371/journal.ppat.0030014 |