دورية أكاديمية
Reusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids
العنوان: | Reusability study of Novozym® 435 for the enzymatic synthesis of mannosyl myristate in pure ionic liquids |
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المؤلفون: | Galonde, N., Richard, G., Deleu, M., Nott, K., Jerôme, C., Fauconnier, ML. |
المصدر: | Biotechnologie, Agronomie, Société et Environnement, Vol 17, Iss 4, Pp 556-562 (2013) |
بيانات النشر: | Presses Agronomiques de Gembloux, 2013. |
سنة النشر: | 2013 |
المجموعة: | LCC:Biotechnology LCC:Environmental sciences |
مصطلحات موضوعية: | Esters, carbohydrates, esterification, lipases, enzyme technology, Biotechnology, TP248.13-248.65, Environmental sciences, GE1-350 |
الوصف: | When developing a biocatalyzed synthesis route, the enzyme reusability is an important parameter to consider for the reduction of industrial costs. In this context, the functional stability of Novozym® 435 in ionic liquids (ILs) was studied in the transesterification of mannose with vinyl myristate. The enzyme was re-used five times in three ILs, 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim][BF4]), 1-butyl-3-methylimidazolium trifluoromethanesulfonate ([Bmim][TFO]), 1-butyl-1-methylpyrrolidinium trifluoromethanesulfonate ([Bmpyrr][TFO]) and in tert-butanol (tert-BuOH). [Bmpyrr][TFO] showed the best 24 h-yield (24 h-η), with 68.8% after the first cycle and the lowest loss of 24 h-η (42%) after five cycles (24 h-η of 39.9%). In comparison with [Bmpyrr][TFO], Novozym® 435 presented the most prominent loss of activity after five cycles of reaction in [Bmim][TFO] (loss of 89%), despite the good 24 h-η obtained after one cycle (60%). [Bmim][BF4] was the least interesting IL, as it was found to lead to the lowest 24 h-η, with 24.5% after one cycle and a significant loss of activity (77%) after five cycles, with a 24 h-η of 5.6%. After five cycles, the 24 h-η in [Bmpyrr][TFO] was higher than in tert-BuOH and the yield loss was higher for the organic solvent (57%). Consequently, these results reveal that, in the present study, the pyrrolidium-based IL [Bmpyrr][TFO] represented the best IL as it allowed the highest level of enzymatic activity and functional stability of Novozym® 435. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English French |
تدمد: | 1370-6233 1780-4507 |
Relation: | http://hdl.handle.net/11006/112; https://doaj.org/toc/1370-6233; https://doaj.org/toc/1780-4507 |
URL الوصول: | https://doaj.org/article/1a6f3d3af13d46679bbfe268000ea272 |
رقم الأكسشن: | edsdoj.1a6f3d3af13d46679bbfe268000ea272 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 13706233 17804507 |
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