دورية أكاديمية
Bombinin-BO1 induces hepatocellular carcinoma cell-cycle arrest and apoptosis via the HSP90A-Cdc37-CDK1 axis
| العنوان: | Bombinin-BO1 induces hepatocellular carcinoma cell-cycle arrest and apoptosis via the HSP90A-Cdc37-CDK1 axis |
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| المؤلفون: | Xiaoting Wang, Peiyu Tang, Yitong Gong, Hang Yao, Mingpeng Liang, Hongxia Qu, Xiao Han, Qiusheng Zheng, Zhen Shi, Defang Li, Qingling Jiang |
| المصدر: | iScience, Vol 27, Iss 8, Pp 110382- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Science |
| مصطلحات موضوعية: | Biological sciences, System biology, Cancer, Science |
| الوصف: | Summary: Bombinin-BO1 (BO1), a bombinin peptide derived from the skin secretion of Bombina orientalis, exhibits broad-spectrum antimicrobial activity. To date, the anticancer effect of BO1 remains unclear. This study confirmed cytotoxicity of BO1 on hepatocellular carcinoma cells by inducing S-phase cycle block and apoptosis. In addition, BO1 was found to be localized in cytoplasm through endocytosis. The combined results of pull down, mass spectrometry, and co-immunoprecipitation suggested that BO1 induced misfolding of CDK1 and degradation by competitively binding HSP90A with Cdc37. It was verified that overexpression of HSP90A in BO1-treated cells significantly inhibited degradation of CDK1. In vivo, BO1 inhibited tumor without being toxic to individuals. This study reveals the anti-tumor mechanism of BO1 in inducing cell-cycle arrest and apoptosis by interfering with HSP90A-Cdc37-CDK1 system. This is the first study to analyze the mechanism of BO1 regulation of tumor cells, providing theoretical basis for BO1 treatment of hepatocellular carcinoma. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2589-0042 43248454 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2589004224016079; https://doaj.org/toc/2589-0042 |
| DOI: | 10.1016/j.isci.2024.110382 |
| URL الوصول: | https://doaj.org/article/c1a8c46e35be432484540b9730d1a60e |
| رقم الأكسشن: | edsdoj.1a8c46e35be432484540b9730d1a60e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25890042 43248454 |
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| DOI: | 10.1016/j.isci.2024.110382 |