دورية أكاديمية
أعرض في EDS

The intestinal virome in children with cystic fibrosis differs from healthy controls.

التفاصيل البيبلوغرافية
العنوان: The intestinal virome in children with cystic fibrosis differs from healthy controls.
المؤلفون: Michael J Coffey, Ivan Low, Sacha Stelzer-Braid, Bernd Wemheuer, Millie Garg, Torsten Thomas, Adam Jaffe, William D Rawlinson, Chee Y Ooi
المصدر: PLoS ONE, Vol 15, Iss 5, p e0233557 (2020)
بيانات النشر: Public Library of Science (PLoS), 2020.
سنة النشر: 2020
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Intestinal bacterial dysbiosis is evident in children with cystic fibrosis (CF) and intestinal viruses may be contributory, given their influence on bacterial species diversity and biochemical cycles. We performed a prospective, case-control study on children with CF and age and gender matched healthy controls (HC), to investigate the composition and function of intestinal viral communities. Stool samples were enriched for viral DNA and RNA by viral extraction, random amplification and purification before sequencing (Illumina MiSeq). Taxonomic assignment of viruses was performed using Vipie. Functional annotation was performed using Virsorter. Inflammation was measured by calprotectin and M2-pyruvate kinase (M2-PK). Eight CF and eight HC subjects were included (50% male, mean age 6.9 ± 3.0 and 6.4 ± 5.3 years, respectively, p = 0.8). All CF subjects were pancreatic insufficient. Regarding the intestinal virome, no difference in Shannon index between CF and HC was identified. Taxonomy-based beta-diversity (presence-absence Bray-Curtis dissimilarity) was significantly different between CF and HC (R2 = 0.12, p = 0.001). Myoviridae, Faecalibacterium phage FP Taranis and unclassified Gokushovirinae were significantly decreased in CF compared with HC (q
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0233557
URL الوصول: https://doaj.org/article/1b4788400dab4b23be75642ad0d102a2
رقم الأكسشن: edsdoj.1b4788400dab4b23be75642ad0d102a2
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0233557