دورية أكاديمية
The Novel Diagnostic Biomarkers for Focal Segmental Glomerulosclerosis
| العنوان: | The Novel Diagnostic Biomarkers for Focal Segmental Glomerulosclerosis |
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| المؤلفون: | Mohsen Nafar, Shiva Kalantari, Shiva Samavat, Mostafa Rezaei-Tavirani, Dorothea Rutishuser, Roman A. Zubarev |
| المصدر: | International Journal of Nephrology, Vol 2014 (2014) |
| بيانات النشر: | Wiley, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Diseases of the genitourinary system. Urology |
| مصطلحات موضوعية: | Diseases of the genitourinary system. Urology, RC870-923 |
| الوصف: | Background. Focal segmental glomerulosclerosis (FSGS) is a glomerular injury with various pathogenic mechanisms. Urine proteome panel might help in noninvasive diagnosis and better understanding of pathogenesis of FSGS. Method. We have analyzed the urine sample of 11 biopsy-proven FSGS subjects, 8 healthy controls, and 6 patients with biopsy-proven IgA nephropathy (disease controls) by means of liquid chromatography tandem mass spectrometry (nLC-MS/MS). Multivariate analysis of quantified proteins was performed by principal component analysis (PCA) and partial least squares (PLS). Results. Of the total number of 389 proteins, after multivariate analysis and additional filter criterion and comparing FSGS versus IgA nephropathy and healthy subjects, 77 proteins were considered as putative biomarkers of FSGS. CD59, CD44, IBP7, Robo4, and DPEP1 were the most significant differentially expressed proteins. These proteins are involved in pathogenic pathways: complement pathway, sclerosis, cell proliferation, actin cytoskeleton remodeling, and activity of TRPC6.There was complete absence of DPEP1 in urine proteome of FSGS subjects compared with healthy and disease controls. DPEP1 acts via leukotrienes on TRPC6 and results in increased podocyte motility and proteinuria. Conclusion. The results suggest a panel of candidate biomarkers for noninvasive diagnosis of FSGS, while complete absence of DPEP1 might represent a novel marker of FSGS. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2090-214X 2090-2158 |
| Relation: | https://doaj.org/toc/2090-214X; https://doaj.org/toc/2090-2158 |
| DOI: | 10.1155/2014/574261 |
| URL الوصول: | https://doaj.org/article/1b7786e767a147d2b2a77cfff77a8e9d |
| رقم الأكسشن: | edsdoj.1b7786e767a147d2b2a77cfff77a8e9d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2090214X 20902158 |
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| DOI: | 10.1155/2014/574261 |