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Kinesin and dynein use distinct mechanisms to bypass obstacles

التفاصيل البيبلوغرافية
العنوان: Kinesin and dynein use distinct mechanisms to bypass obstacles
المؤلفون: Luke S Ferro, Sinan Can, Meghan A Turner, Mohamed M ElShenawy, Ahmet Yildiz
المصدر: eLife, Vol 8 (2019)
بيانات النشر: eLife Sciences Publications Ltd, 2019.
سنة النشر: 2019
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: kinesin, dynein, intracellular transport, Medicine, Science, Biology (General), QH301-705.5
الوصف: Kinesin-1 and cytoplasmic dynein are microtubule (MT) motors that transport intracellular cargoes. It remains unclear how these motors move along MTs densely coated with obstacles of various sizes in the cytoplasm. Here, we tested the ability of single and multiple motors to bypass synthetic obstacles on MTs in vitro. Contrary to previous reports, we found that single mammalian dynein is highly capable of bypassing obstacles. Single human kinesin-1 motors fail to avoid obstacles, consistent with their inability to take sideways steps on to neighboring MT protofilaments. Kinesins overcome this limitation when working in teams, bypassing obstacles as effectively as multiple dyneins. Cargos driven by multiple kinesins or dyneins are also capable of rotating around the MT to bypass large obstacles. These results suggest that multiplicity of motors is required not only for transporting cargos over long distances and generating higher forces, but also for maneuvering cargos on obstacle-coated MT surfaces.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
Relation: https://elifesciences.org/articles/48629; https://doaj.org/toc/2050-084X
DOI: 10.7554/eLife.48629
URL الوصول: https://doaj.org/article/1bdc16fa8ca74213895e60e04b480d23
رقم الأكسشن: edsdoj.1bdc16fa8ca74213895e60e04b480d23
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
DOI:10.7554/eLife.48629